EEA1 antibody (N-Term)

Catalog No. ABIN2779263

Product Details anti-EEA1 Antibody

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Sequence: LTENLLKKEQ DYTKLEEKHN EESVSKKNIQ ATLHQKDLDC QQLQSRLSAS

Predicted Reactivity: Cow: 92%, Dog: 75%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against EEA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EEA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1411 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EEA1 antibody (ABIN2779263)

Cebrian, Croce, Guerrero, Blanchard, Mayorga: "Rab22a controls MHC-I intracellular trafficking and antigen cross-presentation by dendritic cells." in: EMBO reports, Vol. 17, Issue 12, pp. 1753-1765, (2016) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1 (EEA1 Products)

Synonyms: MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody

Background: EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
Alias Symbols: MST105, ZFYVE2, MSTP105
Protein Interaction Partner: UBC, RNF2, BMI1, CFTR, ERBB2, VCP, EEA1, RAB5A, TNK2, RABEP1, RABGEF1, PDCD6IP, PXK, HDAC6, ZFYVE16, USO1, STX6, RAB22A, STX12, TOM1, RAB5C, MAPK14, RAB5B, IGF1R, NSF,
Protein Size: 1411

Molecular Weight: 162 kDa

Gene ID: 8411

NCBI Accession: NM_003566, NP_003557

UniProt: Q15075

Pathways: Toll-Like Receptors Cascades