MAP-2 (AA 19-219) antibody
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|2 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Cross-Reactivity||Rat (Rattus), Mouse (Murine)|
|Description||Microtubule-associated proteins (MAPs) play a crucial role in the development and structure of nerve cells. These proteins are important for the assembly and stability of microtubules during neurite outgrowth and for the morphology of neuronal processes, such as dendrites, MAP2, specifically localized in dendrites, has four known isoforms produced by alternative splicing of the transcript. These isoforms, MAPs A, B, C, and D, are expressed at various stages of neuronal development. MAP2B is a 280-kDa protein expressed throughout brain development. It is composed of several highly conserved domains that are flanked by domains with extensive sequence divergence. An N-terminal conserved domain overlaps with a binding domain for the regulatory subunit of the cAMP-dependent kinase II, while a C-terminal conserved domain overlaps with a microtubule-binding domain. Secondary structure prediction suggests that the portion of MAP2B extending from the microtubule surface is composed of a number of alpha-helices separated by small turns which may account for the extended, yet flexible, structure of MAP2B.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||280 kDa|
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Kindler, Schulz, Goedert et al.: "Molecular structure of microtubule-associated protein 2b and 2c from rat brain." in: The Journal of biological chemistry, Vol. 265, Issue 32, pp. 19679-84, 1991 (PubMed).
Kanaani, el-Husseini, Aguilera-Moreno et al.: "A combination of three distinct trafficking signals mediates axonal targeting and presynaptic clustering of GAD65." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1229-38, 2002 (PubMed).