SNAP91 antibody (AA 706-896)

Catalog No. ABIN967993

Product Details anti-SNAP91 Antibody

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Binding Specificity: AA 706-896

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP91 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat AP180 aa. 706-896

Clone: 34-AP180

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SNAP91 antibody (ABIN967993)

Kalthoff, Groos, Kohl, Mahrhold, Ungewickell: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, (2002) (PubMed).

Drake, Traub: "Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28700-9, (2001) (PubMed).

Traub, Downs, Westrich, Fremont: "Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 16, pp. 8907-12, (1999) (PubMed).

Ye, Lafer: "Clathrin binding and assembly activities of expressed domains of the synapse-specific clathrin assembly protein AP-3." in: The Journal of biological chemistry, Vol. 270, Issue 18, pp. 10933-9, (1995) (PubMed).

Morris, Schröder, Plessmann, Weber, Ungewickell: "Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site." in: The EMBO journal, Vol. 12, Issue 2, pp. 667-75, (1993) (PubMed).

Target Details for SNAP91

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Alternative Name: AP180 (SNAP91 Products)

Synonyms: AP180 antibody, CALM antibody, Ap180 antibody, 91kDa antibody, F1-20 antibody, mKIAA0656 antibody, synaptosome associated protein 91 antibody, synaptosomal-associated protein, 91kDa homolog antibody, synaptosomal-associated protein 91 antibody, SNAP91 antibody, LOC100147907 antibody, Snap91 antibody

Background: AP180, also known as AP-3, F1-20, NP185, and pp155, is one of four assembly proteins which are involved in the organization and assembly of clathrin triskelia in clathrin-coated vesicles. Two of these proteins, AP-1 and AP-2, exist as tetramers, while AP180 and auxilin are monomeric assembly proteins. The clathrin binding potential of AP180 is defined by several regions of the molecule, including the 30 kDa N-terminal domain, a central domain, and a 58 kDa C-terminal domain. Although clathrin binding occurs throughout the molecule, only the C-terminal domain is associated with both binding of clathrin and assembly of clathrin cages. The highly acidic central domain, which contains an uncharged alanine-rich segment, is thought to impart the irregular physical properties to this protein. AP180 is the only clathrin assembly protein specific for synapses and is thought to be involved in synaptic vesicle biogenesis and recycling. Furthermore, AP180 is bound by inositol-6-phosphate which has been shown to be closely regulated in neuronal cells by external stimuli.
Synonyms: AP-3, F1-20, NP185, and pp155

Molecular Weight: 180 kDa