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Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) (AA 254-497) antibody

Details for Product No. ABIN967998, Supplier: Log in to see
Antigen
  • ima2
  • 2410044B12Rik
  • IPOA1
  • PTAC58
  • Rch1
  • importin
  • QIP2
  • RCH1
  • SRP1alpha
  • AW146299
  • Gm1055
Epitope
AA 254-497
17
13
5
4
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Fly (Calliphora), Human, Mouse (Murine), Rat (Rattus)
78
28
25
5
2
2
1
1
Host
Mouse
48
15
10
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
69
32
26
17
9
8
6
2
1
1
1
Supplier
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Immunogen Human Rch-1 aa. 254-497
Clone 2-Karyopherin alpha
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Fruit Fly (Drosophila melanogaster)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Karyopherin alpha 2 (KPNA2 Antibody Abstract)
Background The two step process of importing proteins into the nucleus involves the binding and interaction of several cytosolic and nuclear pore proteins. Proteins to be translocated into the nucleus contain a nuclear localization sequence (NLS) which is recognized and bound by carrier proteins in the cytosol. Heterodimers belonging to a highly conserved family of proteins called karyopherins are required for successful nuclear localization of cytosolic proteins. The alpha-subunits appear to function in the binding of NLS (both simple and bitartite NLS motifs), but both alpha- and beta-subunits are required for successful docking to the nuclear envelope. ATP is required for complete translocation of proteins into the nucleus. Karyopherin alpha2 was first identified as Rch-1, an NLS receptor which interacts with the RAG-1 recombination-activating protein in developing B and T cells. Rch-1 has been reported to be 44% identical to karyopherin alpha1 (hSRP-1 /NPI-1). This antibody is routinely tested by western blot analysis.
Synonyms: Rch-1
Molecular Weight 58 kDa
Pathways
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) (AA 254-497) antibody (ABIN967998) Western blot analysis of Karyopherin alpha on a HeLa cell lysate (Human cervical epit...
Immunofluorescence (IF) image for anti-Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2) (AA 254-497) antibody (ABIN967998) Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).
Product cited in: Grozinger, Schreiber: "Regulation of histone deacetylase 4 and 5 and transcriptional activity by 14-3-3-dependent cellular localization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 14, pp. 7835-40, 2000 (PubMed).

Weis, Mattaj, Lamond: "Identification of hSRP1 alpha as a functional receptor for nuclear localization sequences." in: Science (New York, N.Y.), Vol. 268, Issue 5213, pp. 1049-53, 1995 (PubMed).

Moroianu, Hijikata, Blobel et al.: "Mammalian karyopherin alpha 1 beta and alpha 2 beta heterodimers: alpha 1 or alpha 2 subunit binds nuclear localization signal and beta subunit interacts with peptide repeat-containing nucleoporins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 14, pp. 6532-6, 1995 (PubMed).

Background publications Cuomo, Kirch, Gyuris et al.: "Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 13, pp. 6156-60, 1994 (PubMed).