Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody

Details for Product No. ABIN968027
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Antigen
Synonyms HS1, CTTNL, AW213261, si:dkey-9a20.6, wu:fk96b08, zgc:154014, HCLS1, hcls1
Epitope
AA 17-190
(19), (10), (7), (6), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(84), (24), (22), (14), (14), (1), (1), (1)
Host
Mouse
(58), (22), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(75), (41), (16), (16), (10), (6), (3), (2), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN968027
Quantity 150 µg
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Immunogen Human HS1
Clone 9
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name HS1
Background HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases. This antibody is routinely tested by western blot analysis.
Molecular Weight 75 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody Western blot analysis of HS1 on human endothelial lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of HS1.
anti-Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody (2) Immunofluorescent staining of HL60 cells with anti-HS1 antibody.
anti-Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody (3) anti-Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1) (AA 17-190) antibody (Image 3)
Product cited in: Takemoto, Furuta, Li et al.: "LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck." in: The EMBO journal, Vol. 14, Issue 14, pp. 3403-14, 1995 (PubMed).

Fukuda, Kitamura, Taniuchi et al.: "Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7302-6, 1995 (PubMed).

Yamanashi, Okada, Semba et al.: "Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3631-5, 1993 (PubMed).

Hosts (58), (22), (5)
Reactivities (84), (24), (22), (14), (14), (1), (1), (1)
Applications (75), (41), (16), (16), (10), (6), (3), (2), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (19), (10), (7), (6), (6), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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