Gephyrin antibody (AA 569-726)

Catalog No. ABIN968051

The Mouse Monoclonal anti-Gephyrin antibody has been validated for WB, IHC, IF and IP. It is suitable to detect Gephyrin in samples from Human, Mouse, Rat and Chicken. There are 4+ publications available.

Quick Overview for Gephyrin antibody (AA 569-726) (ABIN968051)

Target: Gephyrin (GPHN)

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Gephyrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 45-Gephyrin

Product Details anti-Gephyrin Antibody

Binding Specificity: AA 569-726

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Gephyrin aa. 569-726

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968546, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Gephyrin antibody (ABIN968051)

Kneussel, Haverkamp, Fuhrmann, Wang, Wässle, Olsen, Betz: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, (2000) (PubMed).

Feng, Tintrup, Kirsch, Nichol, Kuhse, Betz, Sanes: "Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1321-4, (1998) (PubMed).

Meyer, Kirsch, Betz, Langosch: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." in: Neuron, Vol. 15, Issue 3, pp. 563-72, (1995) (PubMed).

Prior, Schmitt, Grenningloh, Pribilla, Multhaup, Beyreuther, Maulet, Werner, Langosch, Kirsch: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." in: Neuron, Vol. 8, Issue 6, pp. 1161-70, (1992) (PubMed).

Target Details for Gephyrin

Target: Gephyrin (GPHN)

Alternative Name: Gephyrin

Background: The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules. This antibody is routinely tested by western blot analysis.

Molecular Weight: 93 kDa

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development