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NOS2 antibody (Nitric Oxide Synthase 2, Inducible) (AA 772-787)

Details for Product anti-NOS2 Antibody No. ABIN968055, Supplier: Log in to see
Antigen
  • NOS2A
  • INOS
  • iNOS
  • INO1
  • IPS
  • IPS 1
  • IPS-1
  • HEP-NOS
  • NOS
  • NOS-II
  • Nos-2
  • Nos2a
  • i-NOS
  • iNos
  • NOS2
  • NOS2a
  • nitric oxide synthase 2, inducible
  • inducible nitric oxide synthase
  • inducible nitric-oxide synthase
  • inositol-3-phosphate synthase 1
  • NOS2
  • INOS
  • LOC100008833
  • nos2
  • LOC396821
  • ISYNA1
  • Nos2
Alternatives
anti-Mouse (Murine) NOS2 antibody for Immunocytochemistry
Epitope
AA 772-787
52
20
15
13
11
10
9
8
8
8
7
7
7
6
5
5
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
257
160
95
15
6
1
1
1
1
Host
Mouse
253
67
Clonality (Clone)
Monoclonal ()
Conjugate
This NOS2 antibody is un-conjugated
13
12
6
5
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
253
157
100
78
73
46
41
32
19
13
13
12
3
1
1
1
1
1
Options
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Immunogen Mouse iNOS aa. 772-787
Clone 2-iNOS
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name iNOS/NOS Type II (NOS2 Antibody Abstract)
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)). iNOS isactivated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-ß affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.
Synonyms: NOS Type II
Molecular Weight 130 kDa
Pathways Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity
Comment

Related Products: ABIN968550, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-NOS2 antibody (Nitric Oxide Synthase 2, Inducible) (AA 772-787) (ABIN968055) Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RA...
Product cited in: Nathan, Xie: "Regulation of biosynthesis of nitric oxide." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 13725-8, 1994 (PubMed).

Koprowski, Zheng, Heber-Katz, Fraser, Rorke, Fu, Hanlon, Dietzschold: "In vivo expression of inducible nitric oxide synthase in experimentally induced neurologic diseases." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 7, pp. 3024-7, 1993 (PubMed).

Vodovotz, Bogdan, Paik, Xie, Nathan: "Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 605-13, 1993 (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).