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Ezrin (EZR) (AA 391-515) antibody

Antigen

Ezrin (EZR)
Synonyms CVL, CVIL, VIL2, MGC1584, FLJ26216, DKFZp762H157, EZR, vil2, wu:fc05c03, wu:fj31d06, cvl, cvil, MGC97499
Binding Site
Alternatives

AA 391-515
Clonality Monoclonal (18)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN968058
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anti-Ezrin (EZR) (AA 391-515) antibody

anti-Ezrin (EZR) (AA 391-515) antibody (2)

Additional Information
Application Details Publications Images Alternatives Variants back to top

Alternative name Ezrin
Immunogen Human Ezrin
Cross-Reactivity Dog (Canine)
Format Liquid
Isotype IgG1
Clone 18
Description First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Molecular Weight 80 kDa
Comments

Related Products: ABIN968619, ABIN967389

Application Details
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Concentration 250 µg/ml
Purity Purified
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative 0.09% Sodium azide.
Storage Store undiluted at -20°C.
Research Area Cancer, Cytoskeleton, Microfilaments
Restrictions For Research Use only

Publications
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Product Berryman, Gary, Bretscher: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, 1996 (PubMed).

Mohler, Kreda, Boucher et al.: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." in: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, 1999 (PubMed).

Defacque, Egeberg, Habermann et al.: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." in: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, 2000 (PubMed).

Anastasiadis, Moon, Thoreson et al.: "Inhibition of RhoA by p120 catenin." in: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, 2001 (PubMed).

Perez, Kinoshita, Hitoshi et al.: "Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, 2002 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Ezrin (EZR)", type "Antibodies"
Hosts Rabbit (107), Mouse (17), Goat (8)
Reactivities Human (131), Mouse (Murine) (80), Rat (Rattus) (68), Dog (Canine) (31), Cow (Bovine) (30), Horse (Equine) (28), Pig (Porcine) (28), Guinea Pig (23), Chicken (5), Hamster (2), Kangaroo (2)
Applications Western Blotting (WB) (101), ELISA (35), Immunofluorescence (IF) (27), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr353 (14), pThr567 (8), pThr566 (6), Internal Region (5), Thr567 (5), C-Term (3), AA 362-585 (2), AA 479-498 (2), N-Term (1), Ser566 (1), Thr566 (1), Tyr566 (1), pThr353 (1), pThr567, pThr564, pThr558 (1), pTyr146 (1), pTyr40 (1), pTyr478 (1)