Ezrin (EZR) (AA 391-515) antibody
|Synonyms||CVL, CVIL, VIL2, MGC1584, FLJ26216, DKFZp762H157, EZR, vil2, wu:fc05c03, wu:fj31d06, cvl, cvil, MGC97499|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Price||Product not available in this region.|
|Description||First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||80 kDa|
Related Products: ABIN968619, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Research Area||Cancer, Cytoskeleton, Microfilaments|
|Restrictions||For Research Use only|
|Western blot analysis of Ezrin on MDCK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Ezrin. Immunofluorescent staining of HeLa cells.|
Berryman, Gary, Bretscher: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, 1996 (PubMed).
Mohler, Kreda, Boucher et al.: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." in: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, 1999 (PubMed).
Defacque, Egeberg, Habermann et al.: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." in: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, 2000 (PubMed).
Anastasiadis, Moon, Thoreson et al.: "Inhibition of RhoA by p120 catenin." in: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, 2001 (PubMed).
Perez, Kinoshita, Hitoshi et al.: "Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, 2002 (PubMed).