anti-Paxillin antibody (PXN) (AA 1-557)

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Antigen
Synonyms CG18061, CG18576, CG31794, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dmel\\CG31794, Dpax, DpaxA, PDLP, dPax, pax, PXN, LOC100220858, AW108311, AW123232, Pax, wu:fw71f12
Alternatives
Epitope
AA 1-557
(65), (57), (29), (28), (24), (17), (15), (15), (13), (12), (10), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(420), (181), (165), (23), (5), (3), (3), (2), (1), (1), (1), (1), (1)
Host
Mouse
(391), (49), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This Paxillin antibody is un-conjugated
(7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(346), (115), (114), (63), (59), (58), (30), (29), (9), (8), (4), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
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Immunogen Chicken Paxillin aa. 1-557
Clone 165-Paxillin
Isotype IgG1
Cross-Reactivity Human, Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Paxillin (PXN Antibody Abstract)
Background Paxillin, a focal adhesion protein, is a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. This is consisent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3 binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Paxillin was initially detected in fibroblasts, and its phosphorylation may be important during neurite extension during differentiation. This antibody is routinely tested by western blot analysis.
Molecular Weight 68 kDa
Research Area Cancer, Extracellular Matrix
Pathways MAPK Signaling, EGFR Signaling Pathway
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968066) Western blot analysis of Paxillin on human endothelial cell lysate. Lane 1: 1: 1000, ...
Immunofluorescence (IF) image for anti-Paxillin antibody (PXN) (AA 1-557) (ABIN968066) Immunofluorescent staining of WI38 cells with anti-paxillin.
Product cited in: Laukaitis, Webb, Donais et al.: "Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells." in: The Journal of cell biology, Vol. 153, Issue 7, pp. 1427-40, 2001 (PubMed).

Müller, Jung, Wied et al.: "Redistribution of glycolipid raft domain components induces insulin-mimetic signaling in rat adipocytes." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4553-67, 2001 (PubMed).

Salgia, Li, Lo et al.: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, 1995 (PubMed).

Background publications Turner, Glenney, Burridge: "Paxillin: a new vinculin-binding protein present in focal adhesions." in: The Journal of cell biology, Vol. 111, Issue 3, pp. 1059-68, 1990 (PubMed).

Catalog No. ABIN968066
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