Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11) (AA 1-177) antibody

Antigen: Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11)

Binding Site: AA 1-177

Clonality: Monoclonal (79)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN968068

Additional Information

Alternative name: PTP1D

Immunogen: Human PTP1D (SHP2)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken, Frog

Format: Liquid

Isotype: IgG1

Clone: 79

Description: SHP2 (also known as PTP1D, Syp, SHPTP2 and PTP2C) is a member of the cytosolic class of protein-tyrosine phosphatases (PTPs). SHP2 has been reported to contain two SH2 domains where both SH2 domains are N-terminal to the PTP catalytic domain. The expression of SHP2 has been reported to be highest in brain, heart, and kidney. The PTPs are thought to function with other protein-tyrosine kinases to maintain intracellular protein phosphotyrosine homeostasis and cell cycle progression. The presence of SH2 domains in SHP2 (PTP1D) has prompted speculation that binding to specific phosphorylated tyrosine residues is key to its function. SHP2 (PTP1D) is tyrosine- phosphorylated and activated in response to stimulation with EGF or PDGF.
Synonyms: PTP1D, Syp, SHPTP2 and PTP2C

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 65-72 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of SHP2 (PTP1D) on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilultion of the mouse anti- SHP2 (PTP1D) antibody. SHP2 has been reported to be observable in a range between 65-72 kD.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

Publications

Product: Freeman, Plutzky, Neel: "Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11239-43, 1993 (PubMed).

Vogel, Lammers, Huang et al.: "Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation." in: Science (New York, N.Y.), Vol. 259, Issue 5101, pp. 1611-4, 1993 (PubMed).

Kontaridis, Liu, Zhang et al.: "Role of SHP-2 in fibroblast growth factor receptor-mediated suppression of myogenesis in C2C12 myoblasts." in: Molecular and cellular biology, Vol. 22, Issue 11, pp. 3875-91, 2002 (PubMed).

Kabat, Borrego, Brooks et al.: "Role that each NKG2A immunoreceptor tyrosine-based inhibitory motif plays in mediating the human CD94/NKG2A inhibitory signal." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 4, pp. 1948-58, 2002 (PubMed).

Duchene, Schanstra, Pecher et al.: "A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40375-83, 2002 (PubMed).