Minichromosome Maintenance Complex Component 2 (MCM2) (AA 725-888) antibody

Antigen: Minichromosome Maintenance Complex Component 2 (MCM2)

Synonyms: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN, KIAA0030, MGC10606, cb737, chunp6905, mcm2, MGC89529

Binding Site: AA 725-888

Clonality: Monoclonal (46)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Catalog no.: ABIN968122

Additional Information

Alternative name: BM28

Immunogen: Human BM28

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 46

Description: BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 125 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of BM28 on human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-BM28 antibody.

Immunofluorescent staining of Human Endothelial cells.

Publications

Product: Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, 1995 (PubMed).

Todorov, Pepperkok, Philipova et al.: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, 1994 (PubMed).

Ishimi, Ichinose, Omori et al.: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, 1996 (PubMed).

Cook, Park, Burke et al.: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, 2002 (PubMed).

Shreeram, Sparks, Lane et al.: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, 2002 (PubMed).