UBE2L3 antibody (AA 15-133)

Catalog No. ABIN968215

This Mouse Monoclonal antibody specifically detects UBE2L3 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog and Frog and has been mentioned in 2+ publications.

Quick Overview for UBE2L3 antibody (AA 15-133) (ABIN968215)

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Reactivity: Human, Mouse, Rat, Dog, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 20-UbcH7

Product Details anti-UBE2L3 Antibody

Binding Specificity: AA 15-133

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human UbcH7 aa. 15-133

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-UBE2L3 antibody (ABIN968215)

Hagglund, Van Sant, Lopez, Roizman: "Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 631-6, (2002) (PubMed).

Shimura, Schlossmacher, Hattori, Frosch, Trockenbacher, Schneider, Mizuno, Kosik, Selkoe: "Ubiquitination of a new form of alpha-synuclein by parkin from human brain: implications for Parkinson's disease." in: Science (New York, N.Y.), Vol. 293, Issue 5528, pp. 263-9, (2001) (PubMed).

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UbcH7

Background: Progression of the mammalian cell cycle is regulated mainly by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an epsilon-NH2-Lys in a target protein. Ubiquitination is a hallmark for rapid protein degradation of the target protein in the proteosome-a cytoplasmic complex of proteases. Several human homologs of the yeast ubiquitin-conjugating enzymes (Ubc) have been identified and found involved in critical processes such as cell cycle progression and apoptosis. UbcH7 was characterized as an E6-AP-interacting protein required for the ubiquitination of p53. E6-AP is the ubiquitin ligase used as bait in the search for interacting proteins required for the proper ubiquitination of cellular targets. UbcH7 interacts with the C-terminal region of E6-AP named the hect domain. Therefore, UbcH7 is another protein, like Ubc9, involved in the degradation and regulation of critical proteins in the cell cycle. This antibody is routinely tested by western blot analysis.
Synonyms: Ubiquitin Conjugating Enzyme H7

Molecular Weight: 17-18 kDa