Paired Box 5 (PAX5) (AA 151-306) antibody

Antigen: Paired Box 5 (PAX5)

Synonyms: BSAP, PAX5, KLP, EBB-1, Pax-5, noi, pax2a, pax2.1, paxzf-b, Pax[Zf-b]

Binding Site: AA 151-306

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN968220

Additional Information

Alternative name: Pax-5

Immunogen: Human Pax-5

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 24

Description: There are at least nine members of the paired box (Pax) gene family whose protein products are transcription factors involved in development. The conserved paired box DNA-binding domain is found in the N-terminal region of Pax proteins. An octomer and homeodomain sequence are conserved in the center of the proteins. The Ser/Thr/Pro-rich region in the C-terminal portion contains a conserved 100 amino acid transactivating domain. One of the best studied Pax family members, Pax 5, is a B cell specific activator protein (BSAP). In the early stages of B cell development, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. Pax-5 has been found to be important in both B cell and nervous system development. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 50 kDa

Comments:

Related Products: ABIN968544, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of Pax-5 on mouse spleen. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Pax-5 antibody.

Immunofluorescent staining on SW-13 cells.

Pax-5 (clone 24) staining on human tonsil. Formalin fixed paraffin section with citrate buffer pretreatment 10X.

Publications

Product: Adams, Dörfler, Aguzzi et al.: "Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis." in: Genes & development, Vol. 6, Issue 9, pp. 1589-607, 1992 (PubMed).

Zwollo, Arrieta, Ede et al.: "The Pax-5 gene is alternatively spliced during B-cell development." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 10160-8, 1997 (PubMed).

Foss, Reusch, Demel et al.: "Frequent expression of the B-cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin's disease provides further evidence for its B-cell origin." in: Blood, Vol. 94, Issue 9, pp. 3108-13, 1999 (PubMed).

Hertel, Zhou, Hamilton-Dutoit et al.: "Loss of B cell identity correlates with loss of B cell-specific transcription factors in Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma." in: Oncogene, Vol. 21, Issue 32, pp. 4908-20, 2002 (PubMed).

Klein, Tu, Stolovitzky et al.: "Transcriptional analysis of the B cell germinal center reaction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2639-44, 2003 (PubMed).