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MutS Homolog 6 (E. Coli) (MSH6) (AA 225-333) antibody

Details for Product No. ABIN968247
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Antigen
Synonyms cb326, wu:fb36c12, LOC100227810, GTBP, GTMBP, HNPCC5, HSAP, p160, AU044881, AW550279, Gtmbp
Epitope
AA 225-333
(28), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(117), (30), (22), (14), (13), (12), (1)
Host
Mouse
(63), (47), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(80), (43), (38), (19), (18), (13), (10), (10), (4), (4), (3), (3), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN968247
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Immunogen Human MSH6
Clone DF5
Isotype IgG1
Cross-Reactivity Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MSH6
Background DNA mismatch repair in bacteria is carried out by the MutL, MutH, and MutS proteins. Initial binding of MutS to the mismatched DNA is followed by binding of the MutH endonuclease and MutL. Together these proteins form a complex that mediates excision repair. Mutations or deficiencies of any of these bacterial proteins results in a mutator phenotype that is characterized by genetic instability. MSH2, MSH3, and MSH6 are human homologs of MutS, while MLH1, PMS1, and PMS2 are homologs of MutL. As a heterodimer with MSH2, MSH6 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes single-base mispairs and insertion/deletion loops. Binding of this complex induces conformational changes in the DNA that lead to the binding of an MLH-PMS1 complex and excision repair. Mutations in the human genes are associated with hereditary nonpolyposis colon cancer (HNPCC), a common hereditary disease in humans. HNPCC is characterized by frequent microsatellite mutations that arise from somatic mutation due to a replication error (RER+) phenotype. This phenotype is analogous to the bacterial system and is directly linked to DNA mismatch repair deficiencies.
Synonyms: GTBP
Molecular Weight 160 kDa
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 225-333) antibody Western blot analysis of MSH6/GTBP on A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-MSH6/GTBP.
anti-MutS Homolog 6 (E. Coli) (MSH6) (AA 225-333) antibody (2) Immunofluorescent staining of C3H10T1/2 cells.
Product cited in: Palombo, Gallinari, Iaccarino et al.: "GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells." in: Science (New York, N.Y.), Vol. 268, Issue 5219, pp. 1912-4, 1995 (PubMed).

Christmann, Kaina: "Nuclear translocation of mismatch repair proteins MSH2 and MSH6 as a response of cells to alkylating agents." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36256-62, 2000 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Humbert, Hermine, Hernandez et al.: "Implication of protein kinase C in the regulation of DNA mismatch repair protein expression and function." in: The Journal of biological chemistry, Vol. 277, Issue 20, pp. 18061-8, 2002 (PubMed).

Kariola, Otway, Lönnqvist et al.: "Two mismatch repair gene mutations found in a colon cancer patient--which one is pathogenic?" in: Human genetics, Vol. 112, Issue 2, pp. 105-9, 2003 (PubMed).

Validation Images
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