PKA C (AA 18-347) antibody

Antigen: PKA C

Binding Site: AA 18-347

Clonality: Monoclonal (5B)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968293

Additional Information

Immunogen: Human PKA[Calpha] subunit

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG2b

Clone: 5B

Description: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. The levels of expression of the different subunits vary according to cell and tissue type.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 40 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PKA[C] on HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of antiPKA[C].

Immunofluorescent staining of HeLa cells.

Publications

Product: Taylor, Buechler, Yonemoto: "cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes." in: Annual review of biochemistry, Vol. 59, pp. 971-1005, 1990 (PubMed).

Orellana, Marfella-Scivittaro: "Distinctive cyclic AMP-dependent protein kinase subunit localization is associated with cyst formation and loss of tubulogenic capacity in Madin-Darby canine kidney cell clones." in: The Journal of biological chemistry, Vol. 275, Issue 28, pp. 21233-40, 2000 (PubMed).

Westphal, Soderling, Alto et al.: "Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold." in: The EMBO journal, Vol. 19, Issue 17, pp. 4589-600, 2000 (PubMed).

Chen, Yu, Lee et al.: "CREB is one component of the binding complex of the Ces-2/E2A-HLF binding element and is an integral part of the interleukin-3 survival signal." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4636-46, 2001 (PubMed).

DeSouza, Reiken, Ondrias et al.: "Protein kinase A and two phosphatases are components of the inositol 1,4,5-trisphosphate receptor macromolecular signaling complex." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39397-400, 2002 (PubMed).