Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (AA 225-401) antibody

Antigen: Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2)

Synonyms: ICH1, NEDD2, CASP-2, ICH-1L, ICH-1L/1S, ICH-1, Nedd2, Caspase-2, xCaspase-2

Binding Site: AA 225-401

Clonality: Monoclonal (35)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968324

Additional Information

Alternative name: Caspase-2

Immunogen: Human ICH-1L

Format: Liquid

Isotype: IgG1

Clone: 35

Description: Caspase-2/ICH-1 is related to the C. elegans cell death gene product CED-3 and its mammalian homologue interleukin-1beta-converting enzyme (ICE). Caspase-2 /ICH-1 was identified from a mouse cDNA library and originally termed NEDD-2. The NEDD-2 mRNA was found to be expressed during early mouse embryonic brain development and subsequently down-regulated in adult neuronal tissue. With the identification of the human NEDD-2 gene, the murine gene was renamed Ich-1 to symbolize Ice and ced-3 homology. Caspase-2/ICH-1 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as Caspase-2 long, or ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named Caspase-2 short, or ICH-1S. Overexpression of ICH-1L induces apoptosis, while over-expression of Ich-1S suppresses Rat-1 cell death induced by serum deprivation. Thus, it appears that Caspase-2/ICH-1 plays an important dual role in programmed cell death.
Synonyms: ICH-1L

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 48 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Apoptosis/Necrosis, Cancer, Autophagy

Restrictions: For Research Use only

Images

Western blot analysis of Caspase-2 on Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Caspase-2.

Immunofluorescent staining of FHS cells.

Publications

Product: Wang, Miura, Bergeron et al.: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994 (PubMed).

Mancini, Machamer, Roy et al.: "Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis." in: The Journal of cell biology, Vol. 149, Issue 3, pp. 603-12, 2000 (PubMed).

Shibata, Hisahara, Hara et al.: "Caspases determine the vulnerability of oligodendrocytes in the ischemic brain." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. 643-53, 2000 (PubMed).

Guo, Srinivasula, Druilhe et al.: "Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13430-7, 2002 (PubMed).

Li, Chen, Sinogeeva et al.: "Arsenic trioxide promotes histone H3 phosphoacetylation at the chromatin of CASPASE-10 in acute promyelocytic leukemia cells." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49504-10, 2002 (PubMed).