Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 461-574) antibody

Antigen: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Synonyms: HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta, Drnt, Hif1b, ESTM42, W08714, KIAA4051, mKIAA4051, D3Ertd557e, Arnt1, ARNT, MGC136664, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, AHA-1

Binding Site: AA 461-574

Clonality: Monoclonal (29)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968355

Additional Information

Alternative name: HIF-1beta

Immunogen: Human HIF-1beta/ARNT1

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 29

Description: The Ah-receptor (AHR) is a ligand activated transcription factor that mediates the biological effects of agonists. AHR dimerizes with a structurally related protein known as ARNT (arylhydrocarbon-receptor nuclear transducer). This heterodimer binds enhancer elements and induces the expression of target genes, specifically those involved in the metabolism of xenobiotics. ARNT1 and ARNT2 are members of the basic-helix-loop-helix-PAS family of heterodimeric transcription factors, which also includes AHR, hypoxia-inducible factor-1alpha (HIF-1alpha), and the Drosophila single-minded protein (Sim). While ARNT2 expression is limited to brain and kidney, ARNT1 exhibits ubiquitous expression. A targeted disruption of the Arnt locus in the mouse yields embryonic stem cells that fail to activate genes that normally respond to low oxygen tension. Arnt -/- embryos do not survive and show defective angiogenesis of the yolk sac and branchial arches, stunted development, and wasting. Thus, in addition to its regulation of xenobiotic metabolism genes, ARNT is thought to induce developmental gene expression resulting in vascularization of the developing embryo.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 95 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of HIF-1beta/ARNT1 on Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-HIF-1beta.

Immunofluorescent staining of A431 cells.

Publications

Product: Drutel, Kathmann, Heron et al.: "Cloning and selective expression in brain and kidney of ARNT2 homologous to the Ah receptor nuclear translocator (ARNT)." in: Biochemical and biophysical research communications, Vol. 225, Issue 2, pp. 333-9, 1996 (PubMed).

Maltepe, Schmidt, Baunoch et al.: "Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT." in: Nature, Vol. 386, Issue 6623, pp. 403-7, 1997 (PubMed).

Jiang, Jiang, Zheng et al.: "Phosphatidylinositol 3-kinase signaling controls levels of hypoxia-inducible factor 1." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 12, Issue 7, pp. 363-9, 2001 (PubMed).

Suzuki, Tomida, Tsuruo: "Dephosphorylated hypoxia-inducible factor 1alpha as a mediator of p53-dependent apoptosis during hypoxia." in: Oncogene, Vol. 20, Issue 41, pp. 5779-88, 2001 (PubMed).

Ambrosini, Nath, Sierra-Honigmann et al.: "Transcriptional activation of the human leptin gene in response to hypoxia. Involvement of hypoxia-inducible factor 1." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34601-9, 2002 (PubMed).