ARNT antibody (C-Term)

Catalog No. ABIN2777195

This Rabbit Polyclonal antibody specifically detects ARNT in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Sheep, Guinea Pig, Horse and Dog.It has been mentioned in 1 publication

Quick Overview for ARNT antibody (C-Term) (ABIN2777195)

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Sheep, Guinea Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARNT Antibody

Binding Specificity: C-Term

Purpose: Arnt Antibody - C-terminal region

Sequence: SNEQHVQPTS AQPSSQPEVF QEMLSMLGDQ SNTYNNEEFP DLTMFPPFSE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against Arnt. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP30979-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ARNT antibody (ABIN2777195)

Kurlak, Mistry, Cindrova-Davies, Burton, Broughton Pipkin: "Human placental renin-angiotensin system in normotensive and pre-eclamptic pregnancies at high altitude and after acute hypoxia-reoxygenation insult." in: The Journal of physiology, Vol. 594, Issue 5, pp. 1327-40, (2016) (PubMed).

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: Arnt

Background:

Background Information: Arnt is required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

Gene Name: Aryl hydrocarbon receptor nuclear translocator

Alternative Symbols: Arnt1

Protein Name: Aryl hydrocarbon receptor nuclear translocator

Molecular Weight: 88kDa

Gene ID: 25242

NCBI Accession: NP_036912

UniProt: P41739

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect