Immunofluorescent staining of SK-N-SH cells (right). Cells were seeded in a 384 well collagen coated Microplates at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol and the anti- Glucocorticoid Receptor antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen)(pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). The image was taken using a 20x objective and merged using the BD AttoVison ™ software.This antibody also stained SH-SY5Y, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols.