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Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 448-460) antibody

Details for Product No. ABIN968458
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Antigen
Synonyms CAMKA, PKCCD, PK2CDD, zgc:112538, zgc:123320, camk2, camk2b
Epitope
AA 448-460
(22), (20), (7), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(76), (66), (45), (9), (6), (6), (6), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1)
Host
Mouse
(75), (45), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF), Immunohistochemistry (IHC)
(121), (64), (38), (34), (26), (24), (23), (6), (4), (3), (2), (2), (2)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN968458
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Immunogen Rat CaM Kinase IIalpha
Clone DF7
Isotype IgG1
Cross-Reactivity Mouse (Murine), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CaM Kinase II
Background Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) is a multifunctional Ser/Thr kinase that regulates a number of cellular functions in response to increased intracellular Ca2+. CaM kinase II is widely distributed, but is predominantly expressed in brain. It is involved in the regulation of neuronal functions such as neurotransmitter synthesis, neurotransmitter release, long-term potentiation, and formation of spatial learning. Neuronal CaM kinase II contains heteromers of two major subunits, alpha and beta, at a ratio of 2:1 and homomers of alpha subunits. Each subunit has N-terminal ATP-binding and catalytic/regulatory domains and a C-terminal association domain. The regulatory domain consists of the autoinhibitory and calmodulin-binding sites. Assembly of the association domains of multiple subunits positions the regulatory domains for intersubunit autophosphorylation. After binding Ca2+/calmodulin, CaM kinase II undergoes rapid autophosphorylation of the alpha and beta subunits, which results in a substantial increase in its affinity for Ca2+/calmodulin.
Synonyms: Ca2+/calmodulin-dependent protein kinase II
Molecular Weight 52 kDa
Research Area Kinases/Phosphatases
Comment

Related Products: ABIN967389, ABIN968545

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 448-460) antibody Western blot analysis of CaM Kinase II on a rat cerebrum lysate (First Panel). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-CaM Kinase II antibody.
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 448-460) antibody (2) Immunohistochemical staining of CaM Kinase II on rat brain (center panel). Formalin-fixed paraffin-embedded section without citrate buffer pretreatment (10X magnification).
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (AA 448-460) antibody (3) Immunofluorescence staining of SK-N-SH cells (Human neuroblastoma, ATCC HTB-11) (Second Panel). Cells were seeded in a collagen coated 384-well imaging plate at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol and the mouse anti-CaM Kinase II antibody. The second step reagent used was Alexa Fluor® 488 goat anti-mouse Ig (Invitrogen) (pseudo colored green). Cell nuclei were counter stained with Hoechst 33342 (pseudo colored blue). Images was taken either on a BD Pathway 855 or 435 Bioimager System with a 20x objective and merged using BD AttoVision™ software. This antibody also stains SH-SY5Y, C6, U87 and U373 cells using both the Triton X-100 and methanol fix/perm protocols.
Product cited in: Hanson, Schulman: "Neuronal Ca2+/calmodulin-dependent protein kinases." in: Annual review of biochemistry, Vol. 61, pp. 559-601, 1992 (PubMed).

Ishida, Fujisawa: "Stabilization of calmodulin-dependent protein kinase II through the autoinhibitory domain." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2163-70, 1995 (PubMed).

Brocke, Chiang, Wagner et al.: "Functional implications of the subunit composition of neuronal CaM kinase II." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22713-22, 1999 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Zong, Ren, Young et al.: "AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 15983-7, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968458
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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