Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody

Details for Product No. ABIN968583
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Antigen
Synonyms ARCND2, PI-PLC, Beta4aa, RATBETA4AA
Epitope
AA 752-961
Reactivity
Human
(1)
Host
Mouse
(1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)
(1), (1)
Pubmed 4 references available
Catalog no. ABIN968583
Quantity 50 µg
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Immunogen Human Phospholipase C beta4
Clone 56
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Fruit Fly (Drosophila melanogaster)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Alexa Fluor is a registered trademark of Molecular Probes, Inc., Eugene, OR.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Phospholipase C beta 4
Background Phospholipase C (PLC) hydrolyzes inositol phospholipids into diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Multiple distinct PLC isoenzymes have been identified and divided into three structural types: alpha, beta, and gamma. This classification is based primarily on the location of the conserved X and Y domains, whose structural integrity is essential for a functional catalytic core. The activation of PLCbeta isoenzymes is uniquely regulated by G protein subunits, while PLCgamma is activated following phosphorylation by protein tyrosine kinases. The beta subfamily of PLC consists of at least four members: beta1, beta2, beta3, and beta4. PLCbeta4 differs from the other members in that it is not activated by G protein betagamma subunits, it is not found in the liver or kidney, and it is inhibited by ribonucleotides. Various isoforms of PLbetaC4 result from alternative splicing or proteolytic cleavage. PLCbeta4 is expressed in retina and brain and knockout mice display ataxia and abnormalities in metabotropic glutamate receptor function in the cerebellum. Thus, PLCbeta4 is primarily found in neuronal tissues where it is thought to be important in neurotransmitter signaling pathways.
Synonyms: PLCbeta4
Molecular Weight 130 kDa
Application Notes Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody Western blot analysis of Phospholipase Cbeta4 on a rat pituitary lysate (left). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Phospholipase Cbeta4 antibody.
anti-Phospholipase C beta 4 (PLCb4) (AA 752-961) antibody (2) Immunofluorescent staining of C6 cells (Rat glioma, ATCC CCL-107) (right). Cells were seeded in a 384-well collagen coated microplate at ~ 6,000 cells per well. After overnight incubation, cells were stained using the Triton-X 100 fix/perm protocol and the mouse anti-Phospholipase Cbeta4 antibody. The second step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Invitrogen). The image was taken on a BD Pathway™ 855 or 435 Bioimager using a 20x objective. This antibody also stained SH-SY5Y (Human neuroblastoma, ATCC CRL-2266) and SK-N-SH cells (Human neuroblastoma, ATCC HTB-11) using both the Triton-X 100 and methanol fix/perm protocols.
Product cited in: Lee, Park, Lee et al.: "Purification, molecular cloning, and sequencing of phospholipase C-beta 4." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21318-27, 1993 (PubMed).

Alvarez, Ghalayini, Xu et al.: "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)." in: Genomics, Vol. 29, Issue 1, pp. 53-61, 1996 (PubMed).

Kim, Jun, Lee et al.: "Phospholipase C isozymes selectively couple to specific neurotransmitter receptors." in: Nature, Vol. 389, Issue 6648, pp. 290-3, 1997 (PubMed).

Kano, Hashimoto, Watanabe et al.: "Phospholipase cbeta4 is specifically involved in climbing fiber synapse elimination in the developing cerebellum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15724-9, 1999 (PubMed).

Hosts (1)
Reactivities (1)
Applications (1), (1)
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