Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody

Antigen: Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)

Synonyms: STAT6B, STAT6C, D12S1644, IL-4-STAT

Binding Site: pTyr641

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968594

Additional Information

Alternative name: Stat6

Immunogen: Phosphorylated Human Stat6 (pY641)

Format: Liquid

Isotype: IgG2a

Clone: 18

Description: Interleukin-4 (IL-4), a major immunoregulatory cytokine, is secreted by activated T lymphocytes, basophils, and mast cells and plays an important role in modulating T helper cell lineage development. It induces specific gene expression via the tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). Stat6, a member of the signal transducers and activators of transcription protein family, mediates signals for IL-4 and, possibly, IL-13. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta. Following cytokine receptor ligation, Jak kinases are activated and phosphorylate the cytoplasmic tails of the oligomerized receptors. The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. Thus, Stat6 mediates the IL-4 signal and is essential for the proper development of adaptive immunity. Immunofluorescent labelling of phosphorylated Stat6 in endothelial cells exposed to 10 ng/ml IL-4 for 1 hour.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 100 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Receptors, Inflammation

Restrictions: For Research Use only

Images

Immunofluorescent labelling of phosphorylated Stat6 in endothelial cells exposed to 10 ng/ml IL-4 for 1 hour.

Human Endothelial cells were either untreated (left column) or treated with (right column) IL-4 (10 ng/ml) for 1 hour at 37°C. The top panel was probed with Stat6 (ABIN968455) and the bottom panel was probed with Stat6 (pY641) (ABIN968671).

anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody (Image 3)

Publications

Product: Quelle, Shimoda, Thierfelder et al.: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).

Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Mikita, Campbell, Wu et al.: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).