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Kinase Suppressor of Ras 1 (KSR1) (AA 90-203) antibody

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Synonyms CG2899, Dmel\\CG2899, EK3-1, KSR, KSR-1, Ksr, Ksr-1, SR3-1, dKsr, l(3)j5E2, AW492498, B-KSR1, D11Bhm183e, D11Bhm184e, RSU2, hb
AA 90-203
(39), (18), (16), (13), (7), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Mouse (Murine), Rat (Rattus)
(84), (46), (22)
(94), (5), (3)
Clonality (Clone)
Monoclonal ()
(7), (7), (7), (6), (6), (6), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(82), (57), (25), (12), (11), (10), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Mouse KSR-1 aa. 90-203
Clone 15-KSR
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name KSR-1 (KSR1 Antibody Abstract)
Background Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T cells leads to recruitment of MEK to a 700 kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction. Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling. This antibody is routinely tested by western blot analysis.
Molecular Weight 115 kDa

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Kinase Suppressor of Ras 1 (KSR1) (AA 90-203) antibody (ABIN968599) Western blot analysis of KSR-1 on a RSV-3T3 lysate. Lane 1: 1:500, lane 2: 1:1000, la...
Product cited in: Stewart, Sundaram, Zhang et al.: "Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5523-34, 1999 (PubMed).

Michaud, Therrien, Cacace et al.: "KSR stimulates Raf-1 activity in a kinase-independent manner." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12792-6, 1998 (PubMed).

Zhang, Yao, Delikat et al.: "Kinase suppressor of Ras is ceramide-activated protein kinase." in: Cell, Vol. 89, Issue 1, pp. 63-72, 1997 (PubMed).

Therrien, Chang, Solomon et al.: "KSR, a novel protein kinase required for RAS signal transduction." in: Cell, Vol. 83, Issue 6, pp. 879-88, 1996 (PubMed).

Catalog No. ABIN968599
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