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Kinase Suppressor of Ras 1 (KSR1) (AA 90-203) antibody

Details for Product No. ABIN968599
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Antigen
Synonyms KSR, RSU2, hb, Ksr, B-KSR1, AW492498, D11Bhm183e, D11Bhm184e, CG2899, Dmel\\CG2899, EK3-1, KSR-1, Ksr-1, SR3-1, dKsr, l(3)j5E2
Epitope
AA 90-203
(19), (17), (12), (7), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(68), (40), (27), (12), (12), (12), (12)
Host
Mouse
(68), (5), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(58), (40), (20), (17), (14), (7), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 50 µg
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Catalog No. ABIN968599
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Immunogen Mouse KSR-1
Clone Ghr5
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name KSR-1
Background Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T cells leads to recruitment of MEK to a 700 kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction. Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling. This antibody is routinely tested by western blot analysis.
Molecular Weight 115 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Kinase Suppressor of Ras 1 (KSR1) (AA 90-203) antibody Western blot analysis of KSR-1 on a RSV-3T3 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- KSR-1 antibody.
Product cited in: Therrien, Chang, Solomon et al.: "KSR, a novel protein kinase required for RAS signal transduction." in: Cell, Vol. 83, Issue 6, pp. 879-88, 1996 (PubMed).

Zhang, Yao, Delikat et al.: "Kinase suppressor of Ras is ceramide-activated protein kinase." in: Cell, Vol. 89, Issue 1, pp. 63-72, 1997 (PubMed).

Michaud, Therrien, Cacace et al.: "KSR stimulates Raf-1 activity in a kinase-independent manner." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12792-6, 1998 (PubMed).

Stewart, Sundaram, Zhang et al.: "Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5523-34, 1999 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968599
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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