SOD2 antibody (AA 114-220)

Catalog No. ABIN968602

This Mouse Monoclonal antibody specifically detects SOD2 in WB and IF. It exhibits reactivity toward Human, Rat, Mouse and Dog and has been mentioned in 5+ publications.

Quick Overview for SOD2 antibody (AA 114-220) (ABIN968602)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 19-MnSOD

Product Details anti-SOD2 Antibody

Binding Specificity: AA 114-220

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse MnSOD aa. 114-220

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968542, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SOD2 antibody (ABIN968602)

Kim, Roe, Chock, Yim: "Transcriptional activation of the human manganese superoxide dismutase gene mediated by tetradecanoylphorbol acetate." in: The Journal of biological chemistry, Vol. 274, Issue 52, pp. 37455-60, (2000) (PubMed).

Ambrosone, Freudenheim, Thompson, Bowman, Vena, Marshall, Graham, Laughlin, Nemoto, Shields: "Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer." in: Cancer research, Vol. 59, Issue 3, pp. 602-6, (1999) (PubMed).

Gilks, Price, Wright, Churg: "Antioxidant gene expression in rat lung after exposure to cigarette smoke." in: The American journal of pathology, Vol. 152, Issue 1, pp. 269-78, (1998) (PubMed).

Gonzalez-Zulueta, Ensz, Mukhina, Lebovitz, Zwacka, Engelhardt, Oberley, Dawson, Dawson: "Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 6, pp. 2040-55, (1998) (PubMed).

Röhrdanz, Kahl: "Alterations of antioxidant enzyme expression in response to hydrogen peroxide." in: Free radical biology & medicine, Vol. 24, Issue 1, pp. 27-38, (1998) (PubMed).

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: MnSOD

Background: Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese (Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2 and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to toxic stimuli is thought to be critical for the survival of a variety of cell types. This antibody is routinely tested by western blot analysis.
Synonyms: Manganese Superoxide Dismutase

Molecular Weight: 25 kDa

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling