Programmed Cell Death 6 Interacting Protein (PDCD6IP) (AA 375-580) antibody
Alternatives Western Blotting (WB)
|2 references available|
|Price||Product not available in this region.|
Apoptosis is a selective process of genetically programmed cell death which occurs during normal cell differentiation and development of multicellular organisms. In vertebrates, T cell and neuronal development are probably the best characterized systems for the study of apoptosis. ALG-2 and ALG-3 (apoptosis-linked genes 2 and 3) were identified as low molecular weight Ca2+-binding proteins essential for apoptosis through the activation of the Fas receptor in T cells. ALG-2 Interacting Protein 1 (AIP1/Alix) is a ubiquitous protein that associates with ALG-2 in the cytosol in a Ca2+ dependent manner. AIP1 is homologous to the yeast protein, BRO1, which has been implicated in Pkc1p- AP kinase signaling. A truncated form of AIP1 protects against serum starvation-, etoposide-, and staurosporine-induced cell death. In addition, the C-terminal proline rich region of AIP1 facilitates interaction with SH3 domain-containing protein expressed in tumorigenic astrocytes (SETA) and this interaction may be important for mediating DNA damage-dependent apoptosis in astrocytes. Thus, AIP1 interacts with ALG-2 or SETA, or both, during activation of cell death pathways in a variety of cell types.
Synonyms: ALG-2 Interacting Protein 1, Alix
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||105 kDa|
Related Products: ABIN967389
|Synonyms||Aip1, Alix, Eig2, C76364, AI480591, AW544830, mKIAA1375, AIP1, ALIX, DAIP1, DmelCG12876, CG12876, ALiX, GB11744, HP95, DRIP4, MGC17003, zgc:63638, wu:fb70d03, wu:fy65h02, PDCD6IP, aip1, alix, hp95, drip4|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of AIP1 on a rat testis lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-AIP1 antibody.|
Vito, Pellegrini, Guiet et al.: "Cloning of AIP1, a novel protein that associates with the apoptosis-linked gene ALG-2 in a Ca2+-dependent reaction." in: The Journal of biological chemistry, Vol. 274, Issue 3, pp. 1533-40, 1999 (PubMed).
Chen, Borinstein, Gillis et al.: "The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes." in: The Journal of biological chemistry, Vol. 275, Issue 25, pp. 19275-81, 2000 (PubMed).
|Hosts||Rabbit (9), Mouse (8)|
|Reactivities||Human (11), Rat (Rattus) (6), Mouse (Murine) (5), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (17), ELISA (13), Immunofluorescence (IF) (10), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunocytochemistry (ICC) (1)|