rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 351-453) antibody

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Antigen
Synonyms
cool1, p50, p85, pak3, pixb, ARHGEF7, BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB, P85spr, Pak3bp, Cool, PIX, betaPix, betaPix-b, betaPix-c, cool-1, mKIAA0142, p8 ... show more
cool1, p50, p85, pak3, pixb, ARHGEF7, BETA-PIX, COOL-1, COOL1, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB, P85spr, Pak3bp, Cool, PIX, betaPix, betaPix-b, betaPix-c, cool-1, mKIAA0142, p85Cool1, p85SPR show less
Alternatives
Epitope
AA 351-453
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
(47), (22), (22)
Host
Mouse
(49), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (4), (3)
Application
Immunofluorescence (IF), Western Blotting (WB)
(47), (35), (12), (3), (3), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
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Immunogen Rat betaPIX aa. 351-453
Clone 23-bPIX
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name beta PIX (ARHGEF7 Antibody Abstract)
Background The activity of PAK family kinases is regulated through interaction with the small GTPases Cdc42 and Rac1. PAKs are activated by the GTP-bound form of Cdc-42 and Rac1, and recruitment of PAKs to focal complexes has been implicated in Cdc42- and Rac1-dependent regulation of focal contact formation. PAK-interacting exchange factor (PIX) was identified in a screen for proteins that bind PAKs. Two forms of PIX have been identified: an 85 kDa protein designated alphaPIX and a 78 kDa protein designated betaPIX. These proteins have 80% identity in their overlapping regions, which include myosin-like, pleckstrin (PH), Dbl (DH), and SH3 domains. In addition, alphaPIX contains a calponin-like domain at the N-terminus. The expression of betaPIX is ubiquitous, while alphaPIX is expressed in heart, muscle, and thymus. PIX can act as a guanine nuleotide exchange factor for Rac1 and co-transfection of betaPIX, Cdc42, and alphaPAK results in increased alphaPAK activity. PIX binding to PAK is required for localization of PAKs to focal complexes and injection of betaPIX leads to rac1-dependent membrane ruffling. Thus, PIX is important for PAK localization and activity during small GTPase-dependent regulation of cell morphology.
Molecular Weight 78 kDa
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 351-453) antibody (ABIN968623) Western blot analysis of betaPIX on PC12 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3...
Image no. 2 for anti-rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7) (AA 351-453) antibody (ABIN968623) MDCK
Product cited in: Turner, Brown, Perrotta et al.: "Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling." in: The Journal of cell biology, Vol. 145, Issue 4, pp. 851-63, 1999 (PubMed).

Bagrodia, Taylor, Jordon et al.: "A novel regulator of p21-activated kinases." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23633-6, 1998 (PubMed).

Manser, Loo, Koh et al.: "PAK kinases are directly coupled to the PIX family of nucleotide exchange factors." in: Molecular cell, Vol. 1, Issue 2, pp. 183-92, 1998 (PubMed).

Oh, Yoo, Jo et al.: "Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion." in: Biochemical and biophysical research communications, Vol. 235, Issue 3, pp. 794-8, 1997 (PubMed).

Catalog No. ABIN968623
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