PC4 and SFRS1 Interacting Protein 1 (PSIP1) (AA 85-188) antibody
|Synonyms||p52, p75, PAIP, DFS70, LEDGF, PSIP2, MGC74712, Dfs70, Psip2, Ledgfa, Ledgfb, AA408851, AU015605, PSIP1, Ledgf, LEDGF/p75, MGC109898, zgc:109898|
Alternatives Western Blotting (WB), BioImaging (BI), Immunofluorescence (IF)
|5 references available|
|Price||Product not available in this region.|
P52/p75 proteins were first identified through their interaction with transcriptional factors and splicing factors. p75 is encoded by exons 1-15 and contains 530 amino acids, while the alternatively spliced p52 is encoded by exons 1-9 and contains 333 amino acids. Both p52 and p75 have strong interactions with the VP16 activation domain and with various components of the general transcriptional machinery. p52 is the more active transcription activator and has also been shown to recruit the pre-mRNA splicing factor ASF/SF to sites of active transcription. Interestingly, p52/p75 has also been identified as Lens Epithelium-Derived Growth Factor (LEDGF). In serum free media, LEDGF stimulates the growth and survival of a variety of cells, such as lens epithelial cells, retinal photoreceptor cells, COS7 cells, skin fibroblasts, and keratinocytes. In retinal photoreceptor cells, LEDGF is found in the nucleus where it promotes resistance to serum starvation and thermal stress. Thus, the LEDGF gene encodes splice variants that may regulate gene transcription and mRNA splicing of a variety of proteins that are essential for cell growth and survival.
Synonyms: Lens Epithelium-Derived Growth Factor
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||75 kDa and/or 52 kDa|
Related Products: ABIN968535, ABIN967389
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Ge, Si, Roeder: "Isolation of cDNAs encoding novel transcription coactivators p52 and p75 reveals an alternate regulatory mechanism of transcriptional activation." in: The EMBO journal, Vol. 17, Issue 22, pp. 6723-9, 1999 (PubMed).
Ge, Si, Wolffe: "A novel transcriptional coactivator, p52, functionally interacts with the essential splicing factor ASF/SF2." in: Molecular cell, Vol. 2, Issue 6, pp. 751-9, 1999 (PubMed).
Singh, Kimura, Chylack et al.: "Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing." in: Gene, Vol. 242, Issue 1-2, pp. 265-73, 2000 (PubMed).
Nakamura, Singh, Kubo et al.: "LEDGF: survival of embryonic chick retinal photoreceptor cells." in: Investigative ophthalmology & visual science, Vol. 41, Issue 5, pp. 1168-75, 2000 (PubMed).
Cherepanov, Maertens, Proost et al.: "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells." in: The Journal of biological chemistry, Vol. 278, Issue 1, pp. 372-81, 2002 (PubMed).
|Hosts||Rabbit (15), Mouse (5), Goat (1)|
|Reactivities||Human (21), Rat (Rattus) (7), Dog (Canine) (4), Mouse (Murine) (4), Cow (Bovine) (3), Monkey (3), Chicken (1)|
|Applications||Western Blotting (WB) (21), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunofluorescence (IF) (3), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), Immunocytochemistry (ICC) (1)|
|Epitopes||C-Term (3), Internal Region (3), AA 225-275 (2), Center (2), N-Term (1)|