EPB41L1 antibody (AA 510-626)

Catalog No. ABIN968676

Product Details anti-EPB41L1 Antibody

Target: EPB41L1 (erythrocyte Membrane Protein Band 4.1-Like 1 (EPB41L1))

Binding Specificity: AA 510-626

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPB41L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Human, Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse 4.1N aa. 510-626

Clone: 4-4-1N

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-EPB41L1 antibody (ABIN968676)

Ye, Hurt, Wu, Fang, Luo, Hong, Blackshaw, Ferris, Snyder: "Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1N." in: Cell, Vol. 103, Issue 6, pp. 919-30, (2001) (PubMed).

Shen, Liang, Walensky, Huganir: "Regulation of AMPA receptor GluR1 subunit surface expression by a 4. 1N-linked actin cytoskeletal association." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 21, pp. 7932-40, (2000) (PubMed).

Walensky, Blackshaw, Liao, Watkins, Weier, Parra, Huganir, Conboy, Mohandas, Snyder: "A novel neuron-enriched homolog of the erythrocyte membrane cytoskeletal protein 4.1." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 15, pp. 6457-67, (1999) (PubMed).

Target Details for EPB41L1

Target: EPB41L1 (erythrocyte Membrane Protein Band 4.1-Like 1 (EPB41L1))

Alternative Name: 4.1N (EPB41L1 Products)

Synonyms: 4.1N antibody, MRD11 antibody, Epb4.1l1 antibody, Epb41l1 antibody, NBL1 antibody, mKIAA0338 antibody, erythrocyte membrane protein band 4.1 like 1 antibody, erythrocyte membrane protein band 4.1-like 1 antibody, EPB41L1 antibody, Epb41l1 antibody

Background: The erythrocyte membrane cytoskeletal protein, 4.1R, is an important structural element of red blood cells. It contains a spectrin-actin-binding domain (SABD) that facilitates spectrin interaction with F-actin and a membrane-binding domain (MBD) that links the cytoskeleton to the plasma membrane via interactions with Band 3 protein. Two homologues of 4.1R, 4.1G and 4.1N, are expressed throughout the body and in neurons, respectively. These 4.1 proteins contain Ca2+-dependent (CD-CaM) and Ca2+-independent calmodulin (CI-CaM) binding sites within the MBD domain, a centrally located SABD, and a conserved C-terminal domain (CTD). 4.1N expression is high throughout the adult brain and lower in heart, kidney, and pancreas. In neurons, 4.1N protein co-localizes with PSD-95 and GluR1 at sites of synaptic contacts and the CTD of 4.1N allows binding to the C-terminus of GluR1. In addition, 4.1N interacts with the PI3-Kinase enhancer (PIKE) protein and translocates to the nucleus along with PIKE in response to NGF treatment. Thus, 4.1N is a neuronal membrane cytoskeletal protein that may be involved with localizaton of proteins such as neurotransmitter receptors and signaling molecules. Predominant isoforms of 4.1 proteins have been reported to be observable between 30-210 kD due to complex alternative splicing events. 4.1N has been reported to be observable in a range of 100-135 kD in neuronal populations. This antibody is routinely tested by western blot analysis.

Molecular Weight: 116 & 100 kDa