NLR Family, Pyrin Domain Containing 2 (NLRP2) (AA 620-732) antibody
|Synonyms||NBS1, PAN1, NALP2, PYPAF2, CLR19.9, FLJ20510, NLRP2|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|4 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken|
Nijmegen Breakage Syndrome (NBS) is characterized by extreme radiation sensitivity and chromosomal instability. The NBS1 gene product, p95/nibrin/NBS1, forms a complex with Rad50 and Mre11. Cells deficient in this complex have problems with DNA double-strand break repair, cell cycle checkpoint control, and telomere length maintenance. NBS1 contains a forkhead-associated domain (FHA) and a breast cancer carboxy-terminal domain (BRCT) in the N-terminal region. Both of these domains have been found in DNA-damage responsive cell cycle checkpoint proteins. The complex containing NBS1, Rad50, and Mre11 possesses manganese-dependent single stranded DNA endonuclease and 3' to 5' exonuclease activities. In addition, NBS1 is required for DNA-damage dependent phosphorylation of Mre11. This phosphorylation may be required for proper nuclear localization of the NBS1-Rad50-Mre11 complex to sites of DNA double-strand breaks. NBS1 interacts directly with telomere repeat binding factor, TRF1, via its C-terminal region, and both NBS1 and Mre11 co-localize with TRF1 at promyelocytic leukemia nuclear bodies. Thus, the NBS1-Rad50-Mre11 complex may be important for both DNA damage repair, and telomere length maintenance.
Synonyms: Nijmegen Breakage Syndrome-1
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||95 kDa|
Related Products: ABIN968537, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Carney, Maser, Olivares et al.: "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response." in: Cell, Vol. 93, Issue 3, pp. 477-86, 1998 (PubMed).
Trujillo, Yuan, Lee et al.: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, 1998 (PubMed).
Dong, Zhong, Chen: "The Nijmegen breakage syndrome protein is essential for Mre11 phosphorylation upon DNA damage." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19513-6, 1999 (PubMed).
Kim, Krasieva, LaMorte et al.: "Specific recruitment of human cohesin to laser-induced DNA damage." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45149-53, 2002 (PubMed).
|Hosts||Rabbit (47), Goat (3), Mouse (1), Sheep (1)|
|Reactivities||Human (49), Mouse (Murine) (24), Rat (Rattus) (21), Horse (Equine) (18), Chimpanzee (2), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (34), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), ELISA (13), Immunoprecipitation (IP) (8), Immunohistochemistry (IHC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (7), AA 1-208 (2), AA 1030-1080 (2), AA 130-180 (2), AA 144-159 (2), AA 97-115 (2)|