C-terminal Binding Protein 1 (CTBP1) (AA 345-441) antibody

Antigen: C-terminal Binding Protein 1 (CTBP1)

Synonyms: BARS, MGC104684, CtBP1-L, CtBP1-S, D4S115h, D5H4S115, D5H4S115E, CtBP3/BARS, Bars, BARS-50, MGC93318, 50-kDaBFA-inducedADP-ribosylatedsubstrate, wu:fi43a11, ctbp1-A, CtBP, bars, xctbp1, MGC79613, CTBP1

Binding Site: AA 345-441

Clonality: Monoclonal (3)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968722

Additional Information

Alternative name: CtBP1

Immunogen: Mouse CtBP1

Cross-Reactivity: Human, Rat (Rattus), Chicken, Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 3

Description: Transcriptional repression is critical for genetic regulation of many cellular proteins, and involves either a transcription regulator that has an intrinsic repressor domain, or a transcription regulator that contains a binding site in the regulatory domain where a corepressor can bind. C-terminal binding proteins (CtBP1 and CtBP2) are a class of corepressors that interact with a subset of transcription factors through a PLDLSL sequence motif. CtBP1 and CtBP2 are 80% homologous, and both are expressed at high levels in embryonic tissues. However, CtBP1 is expressed higher in adult tissues, and is more widely expressed in embryonic and adult tissues. Both CtBP1 and CtBP2 bind the deltaEF1 zinc finger-homeodomain transcription factor, and enhance transcriptional repression via interaction with the PLDLSL-sequence in deltaEF1. CtBP1 and CtBP2 bind other zinc finger transcription factors, including Kheper and BKLF. In addition, CtBP2 interacts with other transcription factors, such as hFOG-2, Evi-1, AREB6, and ZEB. Thus, CtBP1 and CtBP2 may be important corepressors for a variety of transcriptional factors. This antibody is routinely tested by western blot analysis.
Synonyms: C-terminal Binding Protein-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 48 kDa

Comments:

Related Products: ABIN967389

Synonyms: BARS, MGC104684, CtBP1-L, CtBP1-S, D4S115h, D5H4S115, D5H4S115E, CtBP3/BARS, Bars, BARS-50, MGC93318, 50-kDaBFA-inducedADP-ribosylatedsubstrate, wu:fi43a11, ctbp1-A, CtBP, bars, xctbp1, MGC79613, CTBP1

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Cancer, Cell Cycle, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of CtBP1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells, ATCC CRL-1443). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-CtBP1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).

Publications

Product: Schaeper, Boyd, Verma et al.: "Molecular cloning and characterization of a cellular phosphoprotein that interacts with a conserved C-terminal domain of adenovirus E1A involved in negative modulation of oncogenic transformation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 23, pp. 10467-71, 1995 (PubMed).

Furusawa, Moribe, Kondoh et al.: "Identification of CtBP1 and CtBP2 as corepressors of zinc finger-homeodomain factor deltaEF1." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8581-90, 2000 (PubMed).

Muraoka, Ichikawa, Shi et al.: "Kheper, a novel ZFH/deltaEF1 family member, regulates the development of the neuroectoderm of zebrafish (Danio rerio)." in: Developmental biology, Vol. 228, Issue 1, pp. 29-40, 2001 (PubMed).

Hildebrand, Soriano: "Overlapping and unique roles for C-terminal binding protein 1 (CtBP1) and CtBP2 during mouse development." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5296-307, 2002 (PubMed).