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TRAF2 and NCK Interacting Kinase (TNIK) (AA 522-644) antibody

Details for Product No. ABIN968788, Supplier: Log in to see
Antigen
  • TNIK
  • 1500031A17Rik
  • 4831440I19Rik
  • AI451411
  • C530008O15Rik
  • C630040K21Rik
  • RGD1561817
Epitope
AA 522-644
37
21
18
17
16
14
6
5
4
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
149
36
29
2
2
2
1
1
Host
Mouse
145
6
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
12
12
12
10
10
10
3
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
83
64
63
31
24
22
7
3
3
3
1
1
1
1
Supplier
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Immunogen Human TNIK aa. 522-644
Clone 53-TNIK
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name TNIK (TNIK Antibody Abstract)
Background Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein kinase) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of the second class lack the GTPase binding sites, but are similar to the former class throughout the catalytic domain. This second class includes germinal center kinase (GCK), HPK, KHS, KRS1 and 2, MST1, 2, and 3, NIK, SOK-1, and TNIK. Traf2- and NCK-interacting kinase (TNIK) is most homologous to another NCK-interacting kinase, NIK. TNIK contains an N- terminal kinase domain, and a C-terminal GCK homology (GCKH) domain. The mRNA of TNIK is expressed highest in heart, brain, and skeletal muscle. Overexpression of TNIK activates the JNK pathway, and leads to the disruption of F-actin structures and the inhibition of cell spreading. In vitro, TNIK can phosphorylate gelsolin. Thus, TNIK is a GCK family kinase that may regulate both the JNK pathway and cytoskeletal dynamics.
Molecular Weight 150 kDa
Research Area Signaling, Extracellular Matrix, Kinases/Phosphatases
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-TRAF2 and NCK Interacting Kinase (TNIK) (AA 522-644) antibody (ABIN968788) Western blot analysis of TNIK of 293 HEK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3...
Product cited in: Fu, Shen, Huang et al.: "TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 43, pp. 30729-37, 1999 (PubMed).