Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) (AA 333-532) antibody

Antigen: Receptor-Interacting Serine-threonine Kinase 2 (RIPK2)

Synonyms: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, RIPK2

Binding Site: AA 333-532

Clonality: Monoclonal (25)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968829

Additional Information

Alternative name: RIP2/RICK

Immunogen: Human RIP2/RICK

Cross-Reactivity: Dog (Canine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 25

Description: Members of the TNFR family (TNFRs, DRs, Fas, lymphotoxin-beta-receptor, CD40, CD30, and OX-40) regulate a variety of cellular responses, such as cell activation, proliferation, differentiation, NF-kappaB activation, and apoptosis. Signaling through TNFR family members involves several families of receptor-associated proteins. RIP and RIP2 (RICK/Cardiak) are ser/thr kinase adaptor molecules that associate with TNFR complexes. Both RIPs contain homologous N-teriminal ser/thr kinase domains, but RIP contains a C-terminal death domain, while RIP2 contains a C-terminal caspase activation and recruitment domain (CARD) similar to those found in IAPs. Both RIP and RIP2 can activate NF-kappaB and cause cell death. RIP2 is recruited to TNFRs through interactions with TRAF1, TRAF5, and TRAF6, and RIP2 activation of NF-kappaB requires IKKalpha, IKKbeta, and IKKgamma. In addition, RIP2 can be activated through interactions with Ras-activated Raf1, and RIP2 can activate ERK1 and ERK2. Thus, RIP proteins may regulate TNFR signaling through both ser/thr kinase activity and interaction with the apoptotic machinery.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 61 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of RIP2/RICK on human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-RIP2/RICK.

Immunofluorescent staining of HeLa cells.

Publications

Product: Inohara, del Peso, Koseki et al.: "RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 20, pp. 12296-300, 1998 (PubMed).

McCarthy, Ni, Dixit: "RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 16968-75, 1998 (PubMed).

Navas, Baldwin, Stewart: "RIP2 is a Raf1-activated mitogen-activated protein kinase kinase." in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33684-90, 1999 (PubMed).

Inohara, Koseki, Lin et al.: "An induced proximity model for NF-kappa B activation in the Nod1/RICK and RIP signaling pathways." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27823-31, 2000 (PubMed).