RuvB-Like 2 (E. Coli) (RUVBL2) (AA 180-228) antibody

Antigen: RuvB-Like 2 (E. Coli) (RUVBL2)

Synonyms: RVB2, TIH2, ECP51, TIP48, CGI-46, INO80J, REPTIN, TIP49B, p47, mp47, MGC144733, MGC144734, reptin, zreptin, wu:fi25f01, MGC52995, ruvbl2, rvb2, ecp51, tip48, cgi-46, tip49b, xReptin, MGC69398, RUVBL2, MGC134565

Binding Site: AA 180-228

Clonality: Monoclonal (42)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968851

Additional Information

Alternative name: TIP49b

Immunogen: Human Reptin 52/TIF-496

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 42

Description: Processes involving DNA transcription, replication and repair are necessary in maintaining normal cell function. For each of these processes, the double stranded NA must first be unwound. This is achieved in an ATP-dependent manner by enzymes termed DNA helicases. Reptin52/TIP49b is a DNA helicase that directly interacts with Pontin52/TIP49a. Reptin52/TIP49b and Pontin52/TIP49a belong to a family of highly conserved proteins that share homology with the bacterial protein RuvB, a helicase involved in double-strand break repair and homologous recombination. Both are TATA binding protein (TBP) interacting proteins that bind to beta-Catenin and are among the 12 polypeptides that comprise the INO80 chromatin-remodeling complex. It has also been demonstrated that Reptin52/TIP49b and Pontin52/TIP49a can be found in a complex with the proto- ncogene c-myc. Reptin52/TIP49b and Pontin52/TIP49a have antagonistic roles as Reptin/TIP49b represses gene activation mediated by beta-Catenin and c-myc, whereas Pontin52/TIP49a stimulates gene activation. Thus, Reptin52/TIP49b and Pontin52/TIP49a are transcriptional co-factors that work together to help control multiple pathways.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 52 kDa

Synonyms: RVB2, TIH2, ECP51, TIP48, CGI-46, INO80J, REPTIN, TIP49B, p47, mp47, MGC144733, MGC144734, reptin, zreptin, wu:fi25f01, MGC52995, ruvbl2, rvb2, ecp51, tip48, cgi-46, tip49b, xReptin, MGC69398, RUVBL2, MGC134565

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of TIP49b on HeLa lysate. Lane 1: 1:500, lane 1: 1:1000, lane 3: 1:2000 dilution of TIP49b.

MDCK

Publications

Product: Kanemaki, Kurokawa, Matsu-ura et al.: "TIP49b, a new RuvB-like DNA helicase, is included in a complex together with another RuvB-like DNA helicase, TIP49a." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22437-44, 1999 (PubMed).

Bauer, Chauvet, Huber et al.: "Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity." in: The EMBO journal, Vol. 19, Issue 22, pp. 6121-30, 2000 (PubMed).

Cho, Bhoumik, Broday et al.: "TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8398-413, 2001 (PubMed).