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beta Actin antibody (Actin, beta) (N-Term)

Details for Product anti-ACTB Antibody No. ABIN968977, Supplier: Log in to see
Antigen
  • A
  • A26C1A
  • A26C1B
  • Ac5C
  • Act
  • act
  • act-2
  • ACT-5
  • Act-5C
  • Act5
  • act 5C
  • ACT5C
  • Act5c
  • act5C
  • ACTA1
  • ACTB
  • actb
  • actba
  • actin
  • Actin
  • Actin/BAP47
  • actin5C
  • Actin5C
  • Actx
  • anon-EST:fe2D2
  • B-actin
  • B-ACTZF
  • Bact
  • bact
  • bactin1
  • bactzf
  • BAP47
  • Bap47
  • beta-actin
  • Beta-actin
  • beta-actin/Bap47
  • BRWS1
  • CG4027
  • cyt5C
  • Dmel\\CG4027
  • E430023M04Rik
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • LOC563571
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • M32055
  • POTE2alpha
  • POTEACTIN
  • ps1tp5bp1
  • PS1TP5BP1
  • T11
  • wu:fd18f05
Epitope
N-Term
161
37
16
16
10
9
7
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Hamster, Rat (Rattus), Monkey
524
252
212
81
61
56
42
39
34
28
25
22
22
17
15
15
15
13
10
10
10
9
7
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Mouse
345
208
18
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This beta Actin antibody is un-conjugated
32
25
17
5
5
4
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
504
221
160
103
95
60
49
40
16
13
12
12
10
8
6
6
3
2
2
2
2
1
1
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino-terminal residues of human beta-Actin conjugated to KLH.
Clone 8H10D10
Isotype IgG2b
Purification Ascites
Alternative Name beta-Actin (ACTB Antibody Abstract)
Background Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Molecular Weight 42 kDa
Gene ID 60
HGNC 60
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 1: Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) ...
Immunocytochemistry (ICC) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 2: Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cell...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (N-Term) (ABIN968977) Figure 3: Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurka...
Product cited in: Rual, Venkatesan, Hao, Hirozane-Kishikawa, Dricot, Li, Berriz, Gibbons, Dreze, Ayivi-Guedehoussou, Klitgord, Simon, Boxem, Milstein, Rosenberg, Goldberg, Zhang, Wong, Franklin, Li, Albala, Lim et al.: "Towards a proteome-scale map of the human protein-protein interaction network. ..." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

Xu, Wratten, Charych, Buyske, Firestein, Brzustowicz: "Increased expression in dorsolateral prefrontal cortex of CAPON in schizophrenia and bipolar disorder." in: PLoS medicine, Vol. 2, Issue 10, pp. e263, 2005 (PubMed).

Pederson, Aebi: "Nuclear actin extends, with no contraction in sight." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5055-60, 2005 (PubMed).

Bruneel, Labas, Mailloux, Sharma, Royer, Vinh, Pernet, Vaubourdolle, Baudin: "Proteomics of human umbilical vein endothelial cells applied to etoposide-induced apoptosis." in: Proteomics, Vol. 5, Issue 15, pp. 3876-84, 2005 (PubMed).