B Lymphoid Tyrosine Kinase (BLK) antibody

Antigen: B Lymphoid Tyrosine Kinase (BLK)

Synonyms: MGC10442, MGC112647, BLK, MGC142356, MGC136231, zgc:136231

Clonality: Monoclonal (1E6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN968983

Additional Information

Alternative name: BLK

Gene ID: 640

Immunogen: Purified recombinant fragment of human BLK expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 1E6

Description: Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development . Activation of B cells by various ligands is accompanied by activation of Blk . It has been suggested that Blk is involved in the control of B cell differentiation and proliferation . Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis . Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response .
Synonyms: MODY11, MGC10442, BLK

Molecular Weight: 58kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Product: Yang, Ng, Zhao et al.: "Population differences in SLE susceptibility genes: STAT4 and BLK, but not PXK, are associated with systemic lupus erythematosus in Hong Kong Chinese." in: Genes and immunity, Vol. 10, Issue 3, pp. 219-26, 2009 (PubMed).

Borowiec, Liew, Thompson et al.: "Mutations at the BLK locus linked to maturity onset diabetes of the young and beta-cell dysfunction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 34, pp. 14460-5, 2009 (PubMed).