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CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence)

Details for Product anti-CBL Antibody No. ABIN968993, Supplier: Log in to see
Antigen
  • 4732447J05Rik
  • C-CBL
  • c-Cbl
  • c-cbl-like
  • cbl
  • Cbl-2
  • CBL2
  • FRA11B
  • NSLL
  • RGD1561386
  • RNF55
Alternatives
anti-Human CBL antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Rat (Rattus)
249
134
128
6
4
4
4
1
1
1
Host
Mouse
202
35
16
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CBL antibody is un-conjugated
7
7
7
7
6
5
5
5
5
5
5
5
4
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
168
70
66
55
55
32
18
16
5
5
4
3
3
1
1
1
1
1
Supplier
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Immunogen Purified recombinant fragment of human C-CBL expressed in E. coli.
Clone 3B12
Isotype IgG1
Purification Ascites
Alternative Name C-CBL (CBL Antibody Abstract)
Background The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Molecular Weight 120 kDa
Gene ID 867
HGNC 867
Pathways TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 2: Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and ne...
Immunocytochemistry (ICC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 3: Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues us...
Immunohistochemistry (IHC) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 6: Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2),...
Western Blotting (WB) image for anti-CBL antibody (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence) (ABIN968993) Figure 7: Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) rec...
Product cited in: Sproul, Xu, Wilhelm, Gire, Greene: "Cbl negatively regulates JNK activation and cell death." in: Cell research, Vol. 19, Issue 8, pp. 950-61, 2009 (PubMed).

Sanada, Suzuki, Shih, Otsu, Kato, Yamazaki, Tamura, Honda, Sakata-Yanagimoto, Kumano, Oda, Yamagata, Takita, Gotoh, Nakazaki, Kawamata, Onodera, Nobuyoshi, Hayashi, Harada, Kurokawa, Chiba, Mori et al.: "Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms. ..." in: Nature, Vol. 460, Issue 7257, pp. 904-8, 2009 (PubMed).

Loh, Sakai, Flotho, Kang, Fliegauf, Archambeault, Mullighan, Chen, Bergstraesser, Bueso-Ramos, Emanuel, Hasle, Issa, van den Heuvel-Eibrink, Locatelli, Stary, Trebo, Wlodarski, Zecca, Shannon et al.: "Mutations in CBL occur frequently in juvenile myelomonocytic leukemia. ..." in: Blood, Vol. 114, Issue 9, pp. 1859-63, 2009 (PubMed).