Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody

Details for Product No. ABIN968993
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Antigen
Synonyms 4732447J05Rik, Cbl-2, c-Cbl, cbl, C-CBL, CBL2, FRA11B, NSLL, RNF55, c-cbl-like, RGD1561386
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(215), (138), (135), (62), (50), (50), (24)
Host
Mouse
(191), (20), (9), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(149), (73), (63), (60), (45), (37), (15), (15), (15), (4), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN968993
396.00 $
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Immunogen Purified recombinant fragment of human C-CBL expressed in E. coli.
Clone 3B12
Isotype IgG1
Purification Ascites
Alternative Name C-CBL
Background Synonyms:
CBL, CBL2, NSLL, C-CBL, RNF55
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Molecular Weight 120 kDa
Gene ID 867
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (2) Figure 6: Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red) .
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (3) Figure 5: Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (4) Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (5) Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (6) Figure 2: Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
anti-Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody (7) Figure 1: Western blot analysis using C-CBL mAb against human C-CBL (AA: 684-865) recombinant protein. (Expected MW is 44.9 kDa)
Product cited in: Sproul, Xu, Wilhelm et al.: "Cbl negatively regulates JNK activation and cell death." in: Cell research, Vol. 19, Issue 8, pp. 950-61, 2009 (PubMed).

Sanada, Suzuki, Shih et al.: "Gain-of-function of mutated C-CBL tumour suppressor in myeloid neoplasms." in: Nature, Vol. 460, Issue 7257, pp. 904-8, 2009 (PubMed).

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