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Cyclin-Dependent Kinase 9 (CDK9) antibody

Antigen

Cyclin-Dependent Kinase 9 (CDK9)
Synonyms TAK, C-2k, CTK1, CDC2L4, PITALRE, CDK9, P-TEF, P-TEFb, PTefb, DmelCG5179, CG5179, wu:fb22e09, wu:fc06c02, MGC95175, cdk9, MGC53831, MGC139578, tak, c-2k, cdc2l4, pitalre, MGC69296
Clonality Monoclonal (1B5A7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
2 references available
Catalog no. ABIN969042
Quantity 0.1ml  (Variants)
Price 396.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CDK9
Gene ID 1025
Immunogen Purified recombinant fragment of human CDK9 expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 1B5A7
Description The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. (provided by RefSeq)Tissue specificity: Ubiquitous.
Synonyms: TAK, C-2k, CTK1, CDC2L4, PITALRE
Molecular Weight 43kDa

Application Details
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Application Notes Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascites
Buffer Ascitic fluid containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Research Area Cell Cycle
Restrictions For Research Use only

Publications
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Product Montanuy, Torremocha, Hernández-Munain et al.: "Promoter influences transcription elongation: TATA-box element mediates the assembly of processive transcription complexes responsive to cyclin-dependent kinase 9." in: The Journal of biological chemistry, Vol. 283, Issue 12, pp. 7368-78, 2008 (PubMed).

Sabò, Lusic, Cereseto et al.: "Acetylation of conserved lysines in the catalytic core of cyclin-dependent kinase 9 inhibits kinase activity and regulates transcription." in: Molecular and cellular biology, Vol. 28, Issue 7, pp. 2201-12, 2008 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Cyclin-Dependent Kinase 9 (CDK9)", type "Antibodies"
Hosts Rabbit (53), Mouse (10)
Reactivities Human (62), Mouse (Murine) (32), Rat (Rattus) (32), Chicken (25), Cow (Bovine) (25), Dog (Canine) (25), Horse (Equine) (18), Pig (Porcine) (11), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)
Applications Western Blotting (WB) (42), ELISA (30), Immunofluorescence (IF) (23), Immunohistochemistry (IHC) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunoprecipitation (IP) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Radioimmunoassay (RIA) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pThr186 (6), pThr29 (6), C-Term (5), N-Term (2), N-Term,C-Term (1), pSer216 (1), pThr170 (1)