USP7 antibody (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated))

Details for Product anti-USP7 Antibody No. ABIN969186, Supplier: Log in to see
Antigen
  • CG1490
  • Dmel\\CG1490
  • USP7
  • 2210010O09Rik
  • AA409944
  • AA617399
  • AU019296
  • AW548146
  • C80752
  • Hausp
  • HAUSP
  • TEF1
  • UBP
  • ubiquitin-specific protease 7
  • ubiquitin specific peptidase 7 (herpes virus-associated)
  • ubiquitin specific peptidase 7
  • Usp7
  • usp7
  • USP7
Reactivity
Human
166
45
42
16
12
10
7
6
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
112
54
1
Clonality (Clone)
Monoclonal ()
Conjugate
This USP7 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
142
63
48
33
26
13
13
11
7
5
3
3
1
Options
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Immunogen Purified recombinant fragment of human HAUSP expressed in E. Coli.
Clone 5F11
Isotype IgG1
Purification Ascites
Alternative Name HAUSP (USP7 Antibody Abstract)
Background USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.
Molecular Weight 128 kDa
Gene ID 7874
HGNC 7874
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
Western Blotting (WB) image for anti-USP7 antibody (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) (ABIN969186) Figure 1: Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate.
Background publications Daubeuf, Singh, Tan, Liu, Federoff, Bowers, Tolba: "HSV ICP0 recruits USP7 to modulate TLR-mediated innate response." in: Blood, Vol. 113, Issue 14, pp. 3264-75, 2009 (PubMed).

Product cited in: Becker, Marchenko, Maurice, Moll: "Hyperubiquitylation of wild-type p53 contributes to cytoplasmic sequestration in neuroblastoma." in: Cell death and differentiation, Vol. 14, Issue 7, pp. 1350-60, 2007 (PubMed).

Lu, Ma, Nguyen, Jones, Oren, Donehower: "The Wip1 Phosphatase acts as a gatekeeper in the p53-Mdm2 autoregulatory loop." in: Cancer cell, Vol. 12, Issue 4, pp. 342-54, 2007 (PubMed).