Hexokinase 2 antibody

Catalog No. ABIN969196

This Mouse Monoclonal antibody specifically detects Hexokinase 2 in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for Hexokinase 2 antibody (ABIN969196)

Target: Hexokinase 2 (HK2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hexokinase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3D3

Product Details anti-Hexokinase 2 Antibody

Purpose: HK2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human HK2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Hexokinase 2 antibody (ABIN969196)

He, Bi, Zheng, Dai, Han, Liang, Ye, Zeng, Zhu, Zhong: "Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 26, Issue 3, pp. 303-8, (2009) (PubMed).

Paudyal, Oriuchi, Paudyal, Higuchi, Nakajima, Endo: "Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography." in: Cancer science, Vol. 99, Issue 2, pp. 260-6, (2008) (PubMed).

Lim, Hao, Shaw, Patel, Szabó, Rual, Fisk, Li, Smolyar, Hill, Barabási, Vidal, Zoghbi: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, (2006) (PubMed).

Target Details for Hexokinase 2

Target: Hexokinase 2 (HK2)

Alternative Name: HK2

Background: The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases .Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Molecular Weight: 102 kDa

UniProt: P52789

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Warburg Effect