KDM3A antibody

Catalog No. ABIN969229

This Mouse Monoclonal antibody specifically detects KDM3A in WB, ELISA and ICC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for KDM3A antibody (ABIN969229)

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDM3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: 2E1

Product Details anti-KDM3A Antibody

Purpose: KDM3A Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human KDM3A expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-KDM3A antibody (ABIN969229)

Sobral, Sechler, Parrish, McCann, Jones, Black, Jedlicka: "KDM3A/Ets1/MCAM axis promotes growth and metastatic properties in Rhabdomyosarcoma." in: Genes & cancer, Vol. 11, Issue 1-2, pp. 53-65, (2020) (PubMed).

Sechler, Parrish, Birks, Jedlicka: "The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis." in: Oncogene, Vol. 36, Issue 29, pp. 4150-4160, (2017) (PubMed).

Hillier, Graves, Fulton, Fulton, Pepin, Minx, Wagner-McPherson, Layman, Wylie, Sekhon, Becker, Fewell, Delehaunty, Miner, Nash, Kremitzki, Oddy, Du, Sun, Bradshaw-Cordum, Ali, Carter, Cordes, Harris et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, (2005) (PubMed).

Nagase, Ishikawa, Suyama, Kikuno, Miyajima, Tanaka, Kotani, Nomura, Ohara: "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 5, Issue 5, pp. 277-86, (1999) (PubMed).

Target Details for KDM3A

Target: KDM3A (Lysine (K)-Specific Demethylase 3A (KDM3A))

Alternative Name: KDM3A

Background: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and alpha-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.

Molecular Weight: 147 kDa

Gene ID: 55818

UniProt: Q9Y4C1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Warburg Effect