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Details for Product No. ABIN969273

Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody

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Antigen
Synonyms ASK1, MEKK5, MAPKKK5, 7420452D20Rik, ASK, Mekk5, MAP3K5, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
Pubmed 1 reference available
Catalog no. ABIN969273
Quantity 100 µL
Price
396.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. coli.
Clone 2-00E-04
Isotype IgG1
Purification Ascites
Alternative Name MAP3K5
Background Synonyms:
ASK1, MEKK5, MAPKKK5
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 AA with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK) /stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Molecular Weight 55 kDa
Gene ID 4217
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Product cited in: Widenmaier, Ao, Kim et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent insulinotropic polypeptide." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009 (PubMed).

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5)", type "Antibodies"
Hosts (262), (15)
Reactivities (276), (123), (72), (36), (24), (13)
Applications (192), (107), (72), (70), (60), (28), (25), (7), (6), (6), (3), (2), (2), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (45), (35), (22), (15), (13), (12), (12), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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