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Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody

Details for Product No. ABIN969273
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Antigen
Synonyms ASK1, MEKK5, MAPKKK5, 7420452D20Rik, ASK, Mekk5, MAP3K5, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11
Reactivity
Human
(313), (131), (76), (36), (24), (13), (1)
Host
Mouse
(298), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(228), (134), (102), (66), (60), (30), (29), (7), (5), (5), (3), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN969273
396.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of human MAP3K5 expressed in E. coli.
Clone 2-00E-04
Isotype IgG1
Purification Ascites
Alternative Name MAP3K5
Background Synonyms:
ASK1, MEKK5, MAPKKK5
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 AA with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK) /stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Molecular Weight 55 kDa
Gene ID 4217
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (2) Figure 4: Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple) .
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (3) Figure 3: Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (4) Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.
anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody (5) Figure 1: Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Product cited in: Widenmaier, Ao, Kim et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent insulinotropic polypeptide." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN969273
396.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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