NF-kappa B p65 antibody

Antigen: NF-kappa B p65

Clonality: Monoclonal (6H7)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN969311

Additional Information

Gene ID: 5970

Immunogen: Purified recombinant fragment of human NF-kappaB p65 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 6H7

Description: Transcription factors of the nuclear factor kappa B (NF-kappaB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-kappaB1 (p105/p50) and NF-kappaB2 (p100/p52). The most studied NF-kappaB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for theinteraction of NF-kappaB with its inhibitor, IkappaB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkappaB. This complex prevents nuclear translocation and activity of NF-kappaB. In response to stimuli such as cytokines, LPS, and viral infections, IkappaB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkappaB/NF-kappaB complex, allowing the free heterodimeric NF-kappaB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the kappaB site within promoters and enhancers and functions as a transcriptional activator.
Synonyms: NFkappaB p65, p65, NFKB3, RELA

Molecular Weight: 65kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Yamit-Hezi, Nir, Wolstein et al.: "Interaction of TAFII105 with selected p65/RelA dimers is associated with activation of subset of NF-kappa B genes." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18180-7, 2000 (PubMed).