OCT4 antibody

Catalog No. ABIN969330

Product Details anti-OCT4 Antibody

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: Synthesized peptide of human Oct4.

Clone: 5F7

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-OCT4 antibody (ABIN969330)

Zhang, Zhang, Wang, Esteban, Pei: "Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35825-33, (2008) (PubMed).

Cha, Sung, Jung, Kim, Lee, Park: "Epigenetic deregulation of the human Oct4 promoter in mouse cells." in: Development genes and evolution, Vol. 218, Issue 10, pp. 561-6, (2008) (PubMed).

Kaltz, Funari, Hippauf, Delorme, Noël, Riminucci, Jacobs, Häupl, Jorgensen, Charbord, Peschel, Bianco, Oostendorp: "In vivo osteoprogenitor potency of human stromal cells from different tissues does not correlate with expression of POU5F1 or its pseudogenes." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 9, pp. 2419-24, (2008) (PubMed).

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct4 (POU5F1 Products)

Synonyms: OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody

Background:

Description: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. (provided by RefSeq).Tissue specificity: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Aliases: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, MGC22487, POU5F1

Molecular Weight: 45 kDa

Gene ID: 5460

HGNC: 5460

Pathways: Stem Cell Maintenance