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Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody

Antigen

Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC)
Synonyms HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, prkdc-A, dna-pkcs, PRKDC, DNA-PKCS
Clonality Monoclonal (3H6)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
2 references available
Catalog no. ABIN969366
Quantity 0.1ml
Price 396.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name PRKDC
Gene ID 5591
Immunogen Purified recombinant fragment of human PRKDC expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 3H6
Description The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity. PRKDC must be bound to DNA to express its catalytic properties.
Synonyms: HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, PRKDC
Molecular Weight 46.9kDa

Application Details
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Application Notes Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascites
Buffer Ascitic fluid containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Huston, Lynch, Mohamed et al.: "EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 12791-6, 2008 (PubMed).

Durkin, Guo, Fryrear et al.: "HTLV-1 Tax oncoprotein subverts the cellular DNA damage response via binding to DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36311-20, 2008 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC)", type "Antibodies"
Hosts Rabbit (92), Mouse (42)
Reactivities Human (127), Mouse (Murine) (45), Dog (Canine) (38), Rat (Rattus) (37), Horse (Equine) (36), Chicken (19), Cow (Bovine) (18), Pig (Porcine) (18), Hamster (3), Xenopus laevis (1)
Applications Western Blotting (WB) (80), Immunofluorescence (IF) (73), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (50), Immunoprecipitation (IP) (36), ELISA (29), Gel Shift (GS) (14), Immunohistochemistry (IHC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Electrophoretic Mobility-Shift Assay (EMSA) (2), Immunoelectron Microscopy (IEM) (2), Functional Studies (Func) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Biotin (5), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
Epitopes pThr2609 (16), Internal Region (4), C-Term (3), Thr2609 (3), AA 4085-4096 (2), pSer2612 (2), AA 1-2713 (1), AA 1850-1900 (1), AA 2050-2100 (1), AA 2100-2150 (1), AA 2713-3198 (1), N-Term (1), pSer2056 (1), pTyr2609 (1)