Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody

Details for Product No. ABIN969366
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Antigen
Synonyms PRKDC, AI326420, AU019811, DNA-PKcs, DNAPDcs, DNAPK, DNPK1, DOXNPH, HYRC1, XRCC7, dxnph, p460, scid, slip, HYRC, p350, dna-pkcs, dnapk, dnpk1, hyrc, hyrc1, prkdc-A, xrcc7, DNA-PKCS
Reactivity
Human
(279), (121), (56), (40), (29), (13), (12), (4), (3), (1), (1), (1)
Host
Mouse
(226), (57), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(15), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
(131), (84), (80), (73), (62), (46), (45), (18), (14), (9), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN969366
396.00 $
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Immunogen Purified recombinant fragment of human PRKDC expressed in E. coli.
Clone 3H6
Isotype IgG1
Purification Ascites
Alternative Name PRKDC
Background Synonyms:
HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, PRkDaC
The PRkDaC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity, PRkDaC must be bound to DNA to express its catalytic properties.
Molecular Weight 46.9 kDa
Gene ID 5591
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody (2) Figure 4: Immunofluorescence analysis of Hela cells using PRKDC mouse mAb (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using PRKDC mouse mAb with DAB staining.
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody (4) Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using PRKDC mouse mAb with DAB staining.
anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) antibody (5) Figure 1: Western blot analysis using PRKDC mAb against HEK293 (1) and PRKDC (AA: 2638-2971) -hIgGFc transfected HEK293 (2) cell lysate.
Product cited in: Huston, Lynch, Mohamed et al.: "EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 12791-6, 2008 (PubMed).

Durkin, Guo, Fryrear et al.: "HTLV-1 Tax oncoprotein subverts the cellular DNA damage response via binding to DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 283, Issue 52, pp. 36311-20, 2008 (PubMed).

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