SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody

Details for Product No. ABIN969404
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Antigen
Synonyms
SBN, fb67b04, mad, madh5, sb:cb400, smd5, wu:fb67b04, XSmad4alpha, dpc4, jip, jv5-1, madh4, smad10, smad5, xsmad4, DWFC, JV5-1, MADH5, 1110051M15Rik, AI451355, Dwf-C, Madh5, MusMLP, SMAD5, DKFZp468M06 ... show more
SBN, fb67b04, mad, madh5, sb:cb400, smd5, wu:fb67b04, XSmad4alpha, dpc4, jip, jv5-1, madh4, smad10, smad5, xsmad4, DWFC, JV5-1, MADH5, 1110051M15Rik, AI451355, Dwf-C, Madh5, MusMLP, SMAD5, DKFZp468M0619 show less
Reactivity
Human, Rat (Rattus)
(73), (33), (31), (14), (13), (13), (1), (1), (1), (1)
Host
Mouse
(54), (24), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(66), (33), (17), (15), (10), (10), (9), (9), (1), (1)
Pubmed 1 reference available
Quantity 100 μL
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Catalog No. ABIN969404
363.00 $
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Immunogen Purified recombinant fragment of human SMAD5 expressed in E. coli.
Clone 3H9
Isotype IgG1
Purification Ascites
Alternative Name SMAD5
Background Synonyms:
Dwfc, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323, SMAD5
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo, lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Molecular Weight 52 kDa
Gene ID 4090
Research Area Organogenesis, Signaling, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody Figure 4: Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple) .
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody (2) Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
anti-SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody (4) Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Product cited in: Chang, Chang, Lee et al.: "Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 10, pp. 3927-32, 2008 (PubMed).

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