SMAD, Mothers Against DPP Homolog 5 (SMAD5) antibody

Antigen: SMAD, Mothers Against DPP Homolog 5 (SMAD5)

Synonyms: Dwfc, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323, Madh5

Clonality: Monoclonal (3H9)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969404

Additional Information

Alternative name: SMAD5

Gene ID: 4090

Immunogen: Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 3H9

Description: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo. lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Synonyms: Dwfc, JV5-1, MADH5, DKFZp781C1895, DKFZp781O1323, SMAD5

Molecular Weight: 52kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Organogenesis, Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Chang, Chang, Lee et al.: "Tumor cell cycle arrest induced by shear stress: Roles of integrins and Smad." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 10, pp. 3927-32, 2008 (PubMed).