SMAD5 antibody (Middle Region)

Catalog No. ABIN3043980

The Rabbit Polyclonal anti-SMAD5 antibody (ABIN3043980) specifically detects SMAD5 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SMAD5 antibody (Middle Region) (ABIN3043980)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SMAD5 Antibody

Binding Specificity: AA 248-267, Middle Region

Purpose: Anti-SMAD5 Antibody

Sequence: IPQIMPSISS RDVQPVAYEE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SMAD5 Antibody (ABIN3043980). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMAD5, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5

Background:

Synonyms: Mothers against decapentaplegic homolog 5,MAD homolog 5,Mothers against DPP homolog 5,JV5-1,SMAD family member 5,SMAD 5,Smad5,hSmad5,SMAD5,MADH5,

Tissue Specificity: Ubiquitous.

Background: Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

Sequence Similarities: Belongs to the dwarfin/SMAD family.

Molecular Weight: 52 kDa

UniProt: Q99717