Recombinant SMAD5 antibody (pSer463, pSer465)

Catalog No. ABIN5557527

This Rabbit Monoclonal antibody specifically detects SMAD5 in WB, IF, ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant SMAD5 antibody (pSer463, pSer465) (ABIN5557527)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMAD5 Antibody

Binding Specificity: pSer463, pSer465

Purpose: phospho-SMAD5 (Ser463 + Ser465) Recombinant Antibody

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S).

Isotype: IgG

Application Details

Application Notes: WB(1:50-200), IHC-P(1:200-400), IHC-F(1:100-500), IF(1:50-200), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: Smad5

Background:

Synonyms: DWFC, JV5-1, MADH5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, SMAD family member 5, SMAD 5, Smad5, hSmad5

Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Gene ID: 4090

UniProt: Q99717