cdk2 (p33, Kinase) antibody

Antigen: cdk2 (p33, Kinase)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN97130

Additional Information

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH). ELISA 1:1,000 - 1:5,000 WESTERN BLOT 1:200 - 1:1,000 IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000 OTHER APPLICATIONS User Optimized

Format: Liquid (sterile filtered)

Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G 1 -S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.

Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. Antiserum will specifically react with a 33 kDa cdk2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk2 from other species may also occur.

Application Details

Application Notes: Suitable for western blotting and other antibody based assays. Multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Use paraffin embedded tissues for immunohistochemistry. Optimal titers for other applications should be determined by the researcher.

Concentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Storage: Store vial at -20° C prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Research Area: Cell Cycle

Restrictions: For Research Use only

Images

Immunoblotting. Rabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 ug of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (ABIN102010) preceded color development.