Glucose-6-Phosphate Dehydrogenase antibody (HRP)

Catalog No. ABIN5596888

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody (HRP)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Leuconostoc mesenteroides, Saccharomyces cerevisiae

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Glucose-6-Phosphate-Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Purification: Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate-Dehydrogenase [Yeast].

Immunogen:

Immunogen: Glucose-6-Phosphate-Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Glucose-6-Phosphate Dehydrogenase has been assayed against 1.0 μg of Glucose-6-Phosphate-Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: Glucose-6-Phosphate Dehydrogenase (G6PD Products)

Synonyms: G6PD1 antibody, g6pd antibody, G6pd antibody, G6pdx antibody, g6pdh antibody, g6pd2 antibody, fj78b06 antibody, wu:fj78b06 antibody, si:dkey-90a13.8 antibody, BA3433 antibody, CORTRD1 antibody, G6PDH antibody, GDH antibody, G28A antibody, G6PD antibody, Gpdx antibody, glucose-6-phosphate dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase antibody, glucose-6-phosphate 1-dehydrogenase (G6PD) antibody, glucose-6-P dehydrogenase antibody, glucose-6-phosphate dehydrogenase L homeolog antibody, glucose-6-phosphate 1-dehydrogenase Zwf antibody, hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase antibody, glucose-6-phosphate dehydrogenase X-linked antibody, G6PD antibody, g6pd antibody, g6pD antibody, zwf antibody, LOC100120232 antibody, LACBIDRAFT_188936 antibody, G6pd antibody, g6pd.L antibody, CNG03280 antibody, Tb10.70.5200 antibody, H6PD antibody, G6pdx antibody, H6pd antibody

Background:

Synonyms: G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Background: Glucose-6-Phosphate Dehydrogenase is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), which maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Cell growth and proliferation are affected by Glucose-6-Phosphate Dehydrogenase. Glucose-6-Phosphate Dehydrogenase inhibitors are under investigation to treat cancers and other conditions. DHEA is a Glucose-6-Phosphate Dehydrogenase inhibitor.

Gene Name: zwf

Gene ID: 6063509

UniProt: P11411

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones